玉米醇溶蛋白/鱼腥草黄酮复合纳米颗粒的制备及胃肠道释放特性
Preparation and gastrointestinal release characteristics of zein/Houttuynia cordata flavonoids composite nanoparticles
蒋雨心 1范方宇 2陈成 3孙雪 1刘炯娜1
作者信息
- 1. 西南林业大学 生命科学学院,云南 昆明 650224
- 2. 西南林业大学 生命科学学院,云南 昆明 650224;西南地区生物多样性保育国家林业和草原局重点实验室,云南 昆明 650224;云南省森林灾害预警与控制实验室,云南 昆明 650224
- 3. 西南林业大学 林学院,云南 昆明 650224
- 折叠
摘要
为提高鱼腥草黄酮(Houttuynia cordata flavonoids,HCF)的稳定性,以玉米醇溶蛋白(Zein)和HCF为原料,采用反溶剂法制备了Zein/HCF复合纳米颗粒,探究了Zein、HCF质量比对复合纳米颗粒的影响,并对其进行了表征.结果表明,HCF与Zein之间存在氢键、疏水及静电相互作用.当m(Zein)∶m(HCF)=20∶3时,制备的Zein/HCF复合纳米颗粒性能最好,其熔融温度最高(85.09℃),比Zein纳米颗粒(72.25℃)增加了12.84℃;其平均粒径、多分散性指数最小、粒径分布最窄、Zeta 电位绝对值及包封率最大,微观结构呈均匀分散的圆球状;其具有较高的 1,1-二苯基-2-三硝基苯肼、2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基清除能力,具有较好的热处理保护能力,在模拟消化过程中,经胃液消化120 min后HCF释放率仅为22.31%,再加入肠液消化120 min后,HCF释放率达到75.66%.HCF在模拟胃肠液中的释放趋势均符合Ritger-Peppas模型,但释放机制不同,胃液中属非Fick扩散机制,肠液中属Fick扩散机制.
Abstract
In order to improve the stability of Houttuynia cordata flavonoids(HCF),Zein/HCF composite nanoparticles were synthesized from zein(Zein)and HCF by anti-solvent method and characterized for structural analysis.The effects of zein and HCF mass ratio on the composite nanoparticles were further investigated.The results showed that there were intermolecular forces,including hydrogen bonding,hydrophobic and electrostatic interactions,between HCF and zein.When m(Zein)∶m(HCF)=20∶3,Zein/HCF composite nanoparticles exhibited the best performance with the highest melting temperature reaching 85.09℃,12.84℃higher than that of Zein nanoparticles(72.25℃),its microstructure uniformly dispersed in a spherical shape with the smallest average particle size and polydispersity index(PDI),the narrowest particle size distribution,and the largest absolute Zeta potential value as well as HCF encapsulation rate.In addition,the composite nanoparticles exhibited higher 1,1-diphenyl-2-picrylhydrazyl and diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)radicals scavenging ability,and better heat treatment protection ability by improving the heating stability of HCF.In the simulated gastrointestinal digestion process,the HCF release rate was only 22.31%after 120 min of digestion by simulated gastric fluid(SGF)and reached 75.66%after 120 min of digestion by simulated intestinal fluid(SIF).The HCF release trends in the simulated gastrointestinal fluid were all consistent with the Ritger-Peppas model,but the release mechanisms were different,with a non-Fick diffusion mechanism in SGF while a Fick diffusion mechanism in SIF.
关键词
鱼腥草黄酮/玉米醇溶蛋白/纳米颗粒/胃肠道释放特性/食品用化学品Key words
Houttuynia cordata flavonoids/zein/nanoparticles/gastrointestinal release characteristics/food chemicals引用本文复制引用
基金项目
云南省教育厅科研项目(2022Y593)
西南林业大学科技创新基金(2021)(KY21003)
云南省"万人计划"青年拔尖人才专项(YNWR-QNBJ-2018-046)
生物学质量工程项目(503190106)
出版年
2024
NETL
NSTL
万方数据