Taking Auricularia auricula residue powder after polysaccharides extracted as raw materials,Auricularia auricula dietary fiber(AADF)was extracted by ultrasound-enzyme-assisted alkali method,and the optimal extraction process was determined by single-factor experiments and Box-Behnken design.The hypoglycemic effect of AADF was further explored by in vivo experiments in mice.The results showed that the extraction rate of AADF reached 69.18%under the optimal process of material liquid ratio(g∶mL)1∶15,ultrasonic power 350 W,ultrasonic time 50 min,and 5.0%α-amylase with high temperature resistance(based on the mass of Auricularia auricula residue powder,the same below).Data from in vivo experiments of diabetic mice with AADF intervention for 28 d indicated that,in comparison with those of the control group treated with normal saline per day,the fasting blood glucose value of the group treated with high dosage AADF[200 mg/(kg·bw)per day]decreased by 13.72%,oral glucose tolerance decreased by 16.95%,the contents of total cholesterol and triglycerides in blood lipids were significantly reduced,and the levels of high-density lipoprotein cholesterol and glutathione peroxidase in serum were increased.The activity of catalase and succinate dehydrogenase in the liver was improved,which was similar to the physiological indexes of the model-positive group[metformin gavage amount of 100 mg/(kg·bw)per day].Histopathological analysis of the liver also demonstrated that AADF showed a certain repair effect on liver injury in diabetic mice.
关键词
黑木耳/膳食纤维/工艺优化/小鼠/降血糖活性/中药现代化技术
Key words
Auricularia auricula/dietary fiber/process optimization/mice/hypoglycemic activity/modern technology of Chinese medicine
维普
万方数据