超声-酶辅助碱法提取黑木耳膳食纤维及对糖尿病小鼠的降血糖作用

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中文摘要：以提取多糖后的黑木耳残渣粉末为原料,采用超声-酶辅助碱法提取黑木耳膳食纤维(AADF),通过单因素实验和Box-Behnken设计确定了AADF的最佳提取工艺,进一步通过小鼠体内实验探究了AADF的降血糖功效.结果表明,在料液比(g∶mL)1∶15、超声功率350 W、超声时间50 min、耐高温α-淀粉酶用量5.0%(以黑木耳残渣粉末的质量为基准,下同)的最佳工艺下,AADF提取率为 69.18%.28 d经AADF干预糖尿病小鼠饮食期间,模型高剂量组[每天AADF灌胃量为200 mg/(kg·bw)]与模型对照组(每天灌胃生理盐水)的糖尿病小鼠相比,空腹血糖值下降13.72%、口服糖耐量降低16.95%,血脂总胆固醇和甘油三酯含量显著降低,高密度脂蛋白胆固醇和血清中谷胱甘肽过氧化物酶的水平升高,肝脏中的过氧化氢酶以及琥珀酸脱氢酶活力得到提高,与模型阳性组[每天二甲双胍灌胃量为100 mg/(kg·bw)]各项生理指标相近;肝脏组织病理学分析也表明,AADF对糖尿病小鼠肝损伤具有一定的修复作用.

外文标题：Ultrasound-enzyme-assisted alkali method for extracting dietary fiber from Auricularia auricula and its hypoglycemic effect on diabetic mice

外文摘要：Taking Auricularia auricula residue powder after polysaccharides extracted as raw materials,Auricularia auricula dietary fiber(AADF)was extracted by ultrasound-enzyme-assisted alkali method,and the optimal extraction process was determined by single-factor experiments and Box-Behnken design.The hypoglycemic effect of AADF was further explored by in vivo experiments in mice.The results showed that the extraction rate of AADF reached 69.18%under the optimal process of material liquid ratio(g∶mL)1∶15,ultrasonic power 350 W,ultrasonic time 50 min,and 5.0%α-amylase with high temperature resistance(based on the mass of Auricularia auricula residue powder,the same below).Data from in vivo experiments of diabetic mice with AADF intervention for 28 d indicated that,in comparison with those of the control group treated with normal saline per day,the fasting blood glucose value of the group treated with high dosage AADF[200 mg/(kg·bw)per day]decreased by 13.72%,oral glucose tolerance decreased by 16.95%,the contents of total cholesterol and triglycerides in blood lipids were significantly reduced,and the levels of high-density lipoprotein cholesterol and glutathione peroxidase in serum were increased.The activity of catalase and succinate dehydrogenase in the liver was improved,which was similar to the physiological indexes of the model-positive group[metformin gavage amount of 100 mg/(kg·bw)per day].Histopathological analysis of the liver also demonstrated that AADF showed a certain repair effect on liver injury in diabetic mice.

外文关键词：

Auricularia auriculadietary fiberprocess optimizationmicehypoglycemic activitymodern technology of Chinese medicine

作者：

杨帆、孙孝帆、李德海、王璐、王楠楠、王鹏

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作者单位：

东北林业大学生命科学学院,黑龙江哈尔滨 150040

黑龙江省森林食品资源利用重点实验室,黑龙江哈尔滨 150040

华迪计算机集团有限公司,北京 100195

关键词：

黑木耳膳食纤维工艺优化小鼠降血糖活性中药现代化技术

基金：

黑龙江省自然科学基金资助项目黑龙江省自然科学基金资助项目黑龙江省高等教育教学改革项目哈尔滨市科技局创新研究基金项目

项目编号：

LH2022C007C2015062SJGY202100212017RAQXJ091

出版年：

2024

DOI：

10.13550/j.jxhg.20230654

精细化工

大连化工研究院设计院中国化工学会精细化工专业委员会辽宁省化工研究院

精细化工

CSTPCD北大核心

影响因子：0.557

ISSN：1003-5214

年,卷(期)：2024.41(8)

参考文献量19