Extraction,physicochemical properties and bioactivities of polysaccharides from Conioselinum vaginatium
Polysaccharides were extracted from Conioselinum vaginatium via ultrasonic-assisted ionic liquid[1-ethyl-3-methylimidazole bromide(EMIMBr)],with the optimal extraction process optimized.The crude polysaccharides of Conioselinum vaginatium(CV-1),with impurities such as protein,pigment and small molecules removed,were characterized by FTIR,SEM and UV-Vis absorption spectroscopy,and were further evaluated for its in vitro antioxidant and hypoglycemic capacities.The results showed that under the optimal extraction process of material-liquid ratio(the ratio of Conioselinum vaginatium dry powder mass to distilled water volume,g∶mL)1∶50,pectinase content(based on the mass of Conioselinum vaginatium dry powder,the same below)0.6%,sonication time 115 min,sonication temperature 55℃,enzyme digestion time 90 min,ionic liquid mass concentration 4 g/L,polysaccharides extracted from Conioselinum vaginatium reached(125.278±0.707)mg/g.CV-1 showed a weight-average relative molecular mass of 17570,and mainly consisting of 5 kinds of monosaccharide(molar fraction):fucose(40.83%),rhamnoose(28.03%),arabinose(18.40%),glucose(6.80%)and mannose(5.94%),but very little protein and uronic acid.Meanwhile,CV-1 was an amorphous and crystalline α-configurational glycoside with trihelical conformation,and displayed a lamellar structure with a flat local surface and a mass loss rate of 81.55%at 550℃.The median inhibition concentrations(IC50)for diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)radical and hydroxyl radical were 1.122 and 1.373 g/L,respectively,and those for α-glucosidase and α-amylase were 14.799 and 15.739 g/L.
Conioselinum vaginatium polysaccharidesextraction processphysicochemical propertiesantioxidant activitieshypoglycemic activitiesmodernization technology of traditional Chinese medicine
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