摘要
采用超声辅助离子液体[1-乙基-3-甲基咪唑溴盐(EMIMBr)]对新疆藁本多糖进行提取,优化了提取工艺.采用 FTIR、SEM、UV-Vis 吸收光谱对新疆藁本多糖经除蛋白、色素和小分子等杂质后得到的新疆藁本粗多糖(CV-1)进行了表征;评价了 CV-1 体外抗氧化能力和降糖能力.结果表明,在料液比(即新疆藁本干粉质量与蒸馏水体积之比,g∶mL)1∶50、果胶酶含量(以新疆藁本干粉质量为基准,下同)0.6%、超声时间115 min、超声温度 55℃、酶解时间 90 min、离子液体质量浓度 4 g/L的最佳提取工艺条件下,新疆藁本多糖提取量为(125.278±0.707)mg/g;CV-1单糖重均相对分子质量为17570,主要含有(摩尔分数)40.83%岩藻糖、28.03%鼠李糖、18.40%阿拉伯糖、6.80%葡萄糖和 5.94%甘露糖;CV-1 是无定形与晶体结构共存的、具有三螺旋构象的α-构型糖苷,且含有极少量的蛋白质和糖醛酸;CV-1呈片状结构,局部表面平整,在550℃时失重率81.55%;CV-1 水溶液对羟基自由基、2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基半数抑制浓度(IC50)分别为1.373、1.122 g/L,对α-葡萄糖苷酶、α-淀粉酶的IC50分别为14.799、15.739 g/L.
Abstract
Polysaccharides were extracted from Conioselinum vaginatium via ultrasonic-assisted ionic liquid[1-ethyl-3-methylimidazole bromide(EMIMBr)],with the optimal extraction process optimized.The crude polysaccharides of Conioselinum vaginatium(CV-1),with impurities such as protein,pigment and small molecules removed,were characterized by FTIR,SEM and UV-Vis absorption spectroscopy,and were further evaluated for its in vitro antioxidant and hypoglycemic capacities.The results showed that under the optimal extraction process of material-liquid ratio(the ratio of Conioselinum vaginatium dry powder mass to distilled water volume,g∶mL)1∶50,pectinase content(based on the mass of Conioselinum vaginatium dry powder,the same below)0.6%,sonication time 115 min,sonication temperature 55℃,enzyme digestion time 90 min,ionic liquid mass concentration 4 g/L,polysaccharides extracted from Conioselinum vaginatium reached(125.278±0.707)mg/g.CV-1 showed a weight-average relative molecular mass of 17570,and mainly consisting of 5 kinds of monosaccharide(molar fraction):fucose(40.83%),rhamnoose(28.03%),arabinose(18.40%),glucose(6.80%)and mannose(5.94%),but very little protein and uronic acid.Meanwhile,CV-1 was an amorphous and crystalline α-configurational glycoside with trihelical conformation,and displayed a lamellar structure with a flat local surface and a mass loss rate of 81.55%at 550℃.The median inhibition concentrations(IC50)for diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)radical and hydroxyl radical were 1.122 and 1.373 g/L,respectively,and those for α-glucosidase and α-amylase were 14.799 and 15.739 g/L.
基金项目
国家自然科学基金项目(82060774)
2022年伊犁师范大学微生物资源保护与开发利用重点实验室项目(YLUKLM2022001)
伊犁师范大学科研创新团队项目(CXZK2021003)
维普
万方数据