The extraction process of flavonoids from Artemisia argyi leaves with ultrasound-assisted ionic liquid was optimized via single factor experiments and response surface analysis,and Artemisia argyi leaves flavonoids obtained was further analyzed for their in vitro antioxidant and hypoglycemic activities.The results showed that under the optimal extraction process of 1-butyl-3-methylimidazolium bromide([C4mim]Br)ethanol water solution with a mass concentration 8 g/L as extraction solvent,material-liquid ratio(g∶mL)of 1∶30 and ultrasonic time of 68 min,the extraction rate of Artemisia argyi leaves flavonoids was 14.36%.Artemisia argyi leaves flavonoids exhibited good scavenging abilities against 1,1-diphenyl-2-trinitrophenylhydrazine radicals(DPPH·),2,2'-diazo-di(3-ethylbenzothiazole-6-sulfonic acid)diamine salts free radicals(ABTS+·)and·OH,and good inhibition activity against α-amylase and α-glucosidase,indicating certain antioxidant and hypoglycemic activities.The clearance rates of Artemisia argyi leaves flavonoids with a mass concentration of 0.5 g/L on DPPH·and ABTS+·were 81.05%and 93.81%,and the median inhibitory concentration(IC50)were 0.15 and 0.12 g/L,respectively.The clearance rate of·OH at Artemisia argyi leaves flavonoids with a mass concentration of 1.0 g/L was 70.28%,and IC50 was 0.60 g/L.The inhibitory rate of α-amylase was 64.70%and IC50 was 0.59 g/L at Artemisia argyi leaves flavonoids with a mass concentration of 1.0 g/L.The inhibitory rate of α-glucosidase was 56.68%and IC50 was 0.37 g/L at Artemisia argyi leaves flavonoids with a mass concentration of 0.5 g/L.
关键词
艾叶/黄酮/离子液体/抗氧化性/降血糖活性/中药现代化技术
Key words
Artemisiae argyi leaves/flavonoids/ionic liquids/antioxidation/hypoglycemic activity/modernization technology of traditional Chinese medicines
维普
万方数据