连翘多糖纳米硒的制备及体外抗氧化和降血糖能力

Preparation,characterization,antioxidant and hypoglycemic ability of polysaccharide nano-selenium from Forsythia suspensa in vitro

姚琳琳 ¹任蕊蕊 ¹李菁岚 ¹孙建瑞 ²王大红²

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作者信息

1. 河南科技大学食品与生物工程学院,河南洛阳 471023
2. 河南科技大学食品与生物工程学院,河南洛阳 471023;河南省食品微生物工程技术研究中心,河南洛阳 471023
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摘要

利用水提醇沉法从连翘中提取了连翘多糖(FP),经硝酸-亚硒酸钠法制备了连翘多糖纳米硒(FP-SeNPs),采用UV-Vis、FTIR、XRD和SEM对其进行了表征.采用1,1-二苯基-2-三硝基苯肼自由基(·DPPH)、羟基自由基(·OH)、2,2-联氮-双(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(·ABTS+)清除实验考察了 FP-SeNPs的体外抗氧化能力.采用α-淀粉酶和α-葡萄糖苷酶活性抑制实验考察了 FP-SeNPs的体外降血糖能力.结果表明,FP-SeNPs中硒含量为908 mg/kg,其形成了颗粒状分布的表面结构,改变了多糖的表面形态,但未破坏多糖的基本结构.FP-SeNPs的UV-Vis吸收光谱在270 nm处出现新峰,其XRD谱图在2θ=20°～30°内出现一个弥散峰,表明FP和SeNPs之间存在相互作用,形成了 FP-SeNPs复合物.FP-SeNPs对·DPPH、·OH和·ABTS+的清除能力和对α-淀粉酶、α-葡萄糖苷酶的活性抑制能力均呈浓度依赖性,质量浓度为1.6 g/L的FP-SeNPs溶液对·DPPH、·OH和·ABTS+的清除率分别为91.98％、56.81％和87.44％,对α-淀粉酶、α-葡萄糖苷酶的活性抑制率分别为55.61％和72.73％,显著高于FP的自由基清除能力和酶活性抑制能力(P＜0.05).

Abstract

Forsythia suspensa polysaccharide(FP)was extracted from dried Forsythia suspensa via water extraction and alcohol precipitation.FP nano-selenium(FP-SENPS)was then prepared through nitric acid-sodium selenite method,and characterized by UV-Vis,FTIR,XRD,and SEM.The FP-SENPs sample was then analyzed by scavenging of 1,1-diphenyl-2-trinitrophenylhydrazine free radicals(·DPPH),hydroxyl free radicals(·OH)and 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)free radicals(·ABTS+)for their in vitro anti-oxidation performance,while by α-amylase and α-glucosidase inhibition assay for their hypoglycemic capacity.The results showed that FP-SeNPs had a selenium content of 908 mg/kg,which formed a surface structure with granular distribution changing the surface morphology of polysaccharide but not destroying the basic structure of polysaccharide.A new UV-Vis adsorption peak for FP-SeNPs was generated at around 270 nm,and a diffuse peak was observed in XRD pattern between 20° and 30°,indicating interaction between FP and SeNPs,and the FP-SeNPs complex.The scavenging ability of FP-SeNPs on·DPPH,·OH,and·ABTS+and the inhibitory ability on α-amylase α-glucosidase were concentration-dependent.The scavenging rates of FP-SeNPs solution with a mass concentration of 1.6 g/L on·DPPH,·OH,and·ABTS+were 91.98％,56.81％,and 87.44％,and the inhibitory rates on α-amylase andα-glucosidase were 55.61％and 72.73％,respectively,significantly higher than those of FP(P＜0.05).

关键词

连翘/多糖/纳米硒/表征/抗氧化活性/降血糖活性/中药现代化技术

Key words

Forsythia suspensa/polysaccharides/nano-selenium/characterization/antioxidant activity/hypoglycemic activity/modernization technology of traditional Chinese medicines

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基金项目

国家自然科学基金项目(31401672)

河南省青年人才托举工程项目(2021HYTP027)

河南省科技攻关项目(232102110134)

出版年

2024

精细化工

大连化工研究院设计院中国化工学会精细化工专业委员会辽宁省化工研究院

精细化工

CSTPCD北大核心

影响因子：0.557

ISSN：1003-5214

参考文献量4

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