Preparation,characterization,antioxidant and hypoglycemic ability of polysaccharide nano-selenium from Forsythia suspensa in vitro
Forsythia suspensa polysaccharide(FP)was extracted from dried Forsythia suspensa via water extraction and alcohol precipitation.FP nano-selenium(FP-SENPS)was then prepared through nitric acid-sodium selenite method,and characterized by UV-Vis,FTIR,XRD,and SEM.The FP-SENPs sample was then analyzed by scavenging of 1,1-diphenyl-2-trinitrophenylhydrazine free radicals(·DPPH),hydroxyl free radicals(·OH)and 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)free radicals(·ABTS+)for their in vitro anti-oxidation performance,while by α-amylase and α-glucosidase inhibition assay for their hypoglycemic capacity.The results showed that FP-SeNPs had a selenium content of 908 mg/kg,which formed a surface structure with granular distribution changing the surface morphology of polysaccharide but not destroying the basic structure of polysaccharide.A new UV-Vis adsorption peak for FP-SeNPs was generated at around 270 nm,and a diffuse peak was observed in XRD pattern between 20° and 30°,indicating interaction between FP and SeNPs,and the FP-SeNPs complex.The scavenging ability of FP-SeNPs on·DPPH,·OH,and·ABTS+and the inhibitory ability on α-amylase α-glucosidase were concentration-dependent.The scavenging rates of FP-SeNPs solution with a mass concentration of 1.6 g/L on·DPPH,·OH,and·ABTS+were 91.98％,56.81％,and 87.44％,and the inhibitory rates on α-amylase andα-glucosidase were 55.61％and 72.73％,respectively,significantly higher than those of FP(P＜0.05).
Forsythia suspensapolysaccharidesnano-seleniumcharacterizationantioxidant activityhypoglycemic activitymodernization technology of traditional Chinese medicines
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维普
