Ultrasound-assisted deep eutectic solvent(DES)was used for extraction of Gardenia flavone,with the extraction process optimized by single factor experiments and orthogonal experiments.The Gardenia flavone obtained was then evaluated for its hypoglycemic effect on human hepatocellular carcinomas(HepG2 cells).The results showed that the water content(based on the volume of DES before adding water,the same below)in DES exhibited the greatest influence on the flavone yield.Under the optimum conditions of n(choline chloride)∶n(1,2-propylene glycol)=1∶3 as extraction solvent,water content 30％,solid-liquid ratio(g∶mL,the same below)1∶33,ultrasound conditions(power 120 W,time 40 min,temperature 60 ℃),the yield of Gardenia flavone reached the highest(32.70 mg/g).Medium(250.0 mg/L)and high(1000.0 mg/L)mass concentrations of Gardenia flavone could significantly reduce the content of triglyceride.Gardenia flavone could promote glucose metabolism by increasing the content of hexokinase and pyruvate kinase in IR-HepG2 cells,reducing the content of triglyceride,and thus achieving hypoglycemic effect.
关键词
深共熔溶剂/栀子黄酮/HepG2细胞/超声辅助提取/降血糖作用/中药现代化技术
Key words
deep eutectic solvents/Gardenia flavone/HepG2 cells/ultrasound-assisted extraction/hyperglycemic effect/modernization technology of traditional Chinese medicines
维普
万方数据