Extraction of Gardenia flavone with ultrasound-assisted deep eutectic solvents and its hypoglycemic effect on HepG2 cells
Ultrasound-assisted deep eutectic solvent(DES)was used for extraction of Gardenia flavone,with the extraction process optimized by single factor experiments and orthogonal experiments.The Gardenia flavone obtained was then evaluated for its hypoglycemic effect on human hepatocellular carcinomas(HepG2 cells).The results showed that the water content(based on the volume of DES before adding water,the same below)in DES exhibited the greatest influence on the flavone yield.Under the optimum conditions of n(choline chloride)∶n(1,2-propylene glycol)=1∶3 as extraction solvent,water content 30％,solid-liquid ratio(g∶mL,the same below)1∶33,ultrasound conditions(power 120 W,time 40 min,temperature 60 ℃),the yield of Gardenia flavone reached the highest(32.70 mg/g).Medium(250.0 mg/L)and high(1000.0 mg/L)mass concentrations of Gardenia flavone could significantly reduce the content of triglyceride.Gardenia flavone could promote glucose metabolism by increasing the content of hexokinase and pyruvate kinase in IR-HepG2 cells,reducing the content of triglyceride,and thus achieving hypoglycemic effect.
deep eutectic solventsGardenia flavoneHepG2 cellsultrasound-assisted extractionhyperglycemic effectmodernization technology of traditional Chinese medicines
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