Effects of Culture Conditions on Callus Induction, Proliferation and the Total Flavonoid Content of Agrimonia pilosa
The cotyledons of Agrimonia pilosa Ledeb were used to study the effect of different culture conditions on callus induction, proliferation and the total flavonoid content through the orthogonal design method. The results showed that: under the condition of 1/2MS + 6 - BA 1.0( mg/L) + NAA 0. 5 (mg/L), at pH 6.5, the callus was induced more easily, and grew well. The condition of 1/2MS + 6 - BA 2.0(mg/L) + NAA 1.0(mg/L) , and pH 6.0 was the most proper condition for callus to proliferate. At the same time, the total flavonoid content in callus was the highest under that condition.
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维普
