A Study on Simultaneous Determination of 7 kinds of Organic Acids and Vc in Dragon Fruit by RP-HPLC
The main factors that affect the separation of organic acids were studied,and a method for sim-ultaneous and rapid determination of purple red flesh dragon fruit in oxalic acid,tartaric acid,malic acid,acetic acid,lactic acid,citric acid,succinic acid and Vc content with reverse-phase high performance liquid chroma-tography(RP-HPLC) was established.The chromatographic conditions are as follows:the column temperature was 30 ℃,the flow rate was 1 mL/min,the wavelength was 215 nm,the buffer was 0.05 mol/L H3 PO4-Na2HPO4 and a Kromasil column (250 mm× 4.6 mm) was adopted.The results showed that malic acid was the most important organic acid,the next was citric acid.Because this method can complete a determination within10 min.So it has the advantages of fast,high sensitivity and good repeatability,it is a high reference to the de-termination and research of metabolism of organic acid and Vc in fruit.
dragon fruitpurple red fleshorganic acidVcreverse-phase high performance liquid chro-matography(RP-HPLC)
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维普
