Effects of Oxytropis glabra DC Poisoning on α-mannosidase of Brain in Hetian Sheep
The aim of this study was to investigate the effects of Oxytropis glabra DC on the content and transcriptional expression of α-mannosidase( AMA) of brain in Hetian sheep,and reveal the toxic mechanism of O.glabra DC.12 Hetian sheep were randomly and equally divided into 3 groups( the control group and exper-imental groupⅠ,Ⅱ) ,the dried plant of O.glabra DC was comminnuted,and the two experimental groups were fed with O.glabra DC at 10 g and 20 g per kg weight everyday respectively during the test period.The sheep consumed the forages freely until typical symptoms were observed.Brain were collected and the contents of AMA and its mRNA expression in different encephalic regions were measured on the 35th day.It was found that AMA in golgi and lysosomal was expressed in brain of all groups, O.glabra DC inhibited the expression of brain AMA1 and AMA2 mRNA,and the difference was obvious.The expression of AMA2 mRNA was not obvious in the brain of experimental groupⅠand the control group( P>0.05) ,the expression of AMA2 mRNA of cerebel-lum in experimental groupⅡwas significantly lower than that in the normal control group(P<0.05),but the expression of AMA2 mRNA was not obvious in experimental groupⅡand the control group in cerebrum,thala-mus,brainstem and hippocampus( P>0.05) .The expression of AMA1 mRNA decreased in experimental groupⅡ,very significant lower than in the control group in cerebellum and thalamus(P<0.01),the expression of AMA1 mRNA decreased in experimental groupⅡ,significant lower than in the control group in cerebrum( P<0.05) ,the expression of AMA1 mRNA of cerebellum in experimental groupⅠwas significantly lower than in the normal control group( P<0.05) ,but the expression of AMA1 mRNA was not obvious in experimental groupⅠand the control group in cerebrum,thalamus,brainstem and hippocampus( P>0.05) .The results showed that O.glabra DC has remarkable effects on the contents of AMA of brain in Hetian sheep.It is indicated that three regions were the effect target sites of O.glabra DC in the brain tissue.It can be concluded that O.glabra DC produces poisoning effect on the contents of AMA and its mRNA expression by promoting cerebellum,cerebrum and thalamus.
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维普
