Functional studies on polygalacturonase gene Fcpg1 in Fusarium commune causing lotus rhizome rot
[Objective]Lotus rhizome rot caused by Fusarium commune seriously affects the production of lotus in China.The polygalacturonase gene Fcpg1 in the pathogen of lotus spoilage was identified,and its role in growth,development and pathogenicity was analyzed,which provided a basis for further research on the pathogenic mechanism of F.commune and the prevention and control of the disease.[Method]BLASTP was used to find Fcpg1 homologous proteins in the whole genome data of F.commune and the amino acid sequence of Fcpg1 was analyzed using online software ExPASy.The Fcpg1 knockout mutant ΔFcpg1 and the complementation strain ΔFcpg1-Com were obtained.Colony morphology,growth rate,spore production,and pathogenicity were measured for the wild-type strain FCN23,the mutant ΔFcpg1,and the complementation strain ΔFcpg1-Com.[Result]The Fcpg1 gene encoded 388 amino acids with a molecular weight of 40.354 ku.The molecular formula was C1756H2775N489O577S12,the isoelectric point was 6.57 and an aliphatic index was 79.9.Fcpg1 was a stable,water-soluble exocytotic protein,mainly localized in the extracellular.Its signal peptide shear site was between amino acids 16-17.The Fcpg1 protein had high homology to the polygalacturonase protein sequence of F.oxysporum.There were no significant differences in colony morphology,vegetative growth,and spore production in the ΔFcpg1 mutant compared with the wild-type strain FCN23 and the complementation strain ΔFcpg1-Com,but the pathogenicity of the mutant ΔFcpg1 was significantly reduced.[Conclusion]Fcpg1 is a typical polygalacturonase protein involved in the regulation of the pathogenicity of lotus rhizome rot.
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