[Objective]This study aims to study the effects of yeast peptide and subtilisin on growth performance,diarrhea rate,and intestinal health of weaned piglets through piglet growth test and diarrhea induction test.[Method]200 healthy weaned piglets of 21-day-old"duroc×landrace×Yorkshire"ternary hybrid were randomly divided into 5 groups,with 5 replicates in each group and 8 replicates each.The basal diet control group,basal diet +300 mg/kg yeast peptide group,basal diet +500 mg/kg yeast peptide group,basal diet +300 mg/kg subtilis peptide group and basal diet +300 mg/kg yeast peptide +300 mg/kg subtilis peptide group were designed respectively.The prefeed period was 7 days,and the formal trial was 28 days.In the induced diarrhea test,30 healthy weaned piglets of 21-day-old"duroc×landrace×Yorkshire"ternary hybrid were randomly divided into 3 groups,with10 piglets in each group.Each group was fed with basal diet control group,basal diet +300 mg/kg yeast peptide group,basal diet +500 mg/kg yeast peptide group,respectively.Prefeed period was 7 d,formal test lasted 8 d.The unsterilized well water were mixed with the diet to induced diarrhea in the first 3 d,and disinfected well water were mixed with the diet in the last 5d.[Result](1)The addition of different doses of yeast peptide and subtilis peptide to the diet in the growth test had no significant effect on the growth performance of weaned piglets(P>0.05).In the induced diarrhea test,yeast peptide dose-dependent reduced the diarrhea rate of piglets;(2)The addition of 500 mg/kg yeast peptide significantly increased the activity of GSH-PX(glutathione peroxidase)in piglet blood;(3)The addition of subtilisin 300 mg/kg significantly increased the height and villi/recess ratio(P<0.05)and the expression of jejunal nexus protein Claudin-1(P<0.05).(4)The addition of yeast peptide 300 mg/kg + subtilisin 300 mg/kg complex peptide could significantly reduce the content of IL-1β(interleukin-1β)in the blood(P<0.05),increased the villi height and crypt depth(P<0.05)of the jejunum,and significantly increased the coverage index(P<0.05),and the Simpson index had a tendency to increase(0.05<P<0.1).[Conclusion]The addition of 500 mg/kg yeast peptide to the feed can effectively prevent the occurrence of diarrhea in piglets,improve the level of immunoglobulin and antioxidant capacity.The addition of 300 mg/kg subtilis peptide alone or a combination of 300 mg/kg yeast peptide and 300 mg/kg subtilis peptide could increase intestinal absorption and barrier function,reduce intestinal inflammation,and improve the diversity of intestinal microorganisms.
关键词
酵母肽/枯草肽/断奶仔猪/腹泻/肠道健康
Key words
yeast peptides/subtilisin/weaned piglets/diarrhoea/gut health
维普
万方数据