Effects of microalgae combined with Bacillus subtilis on soil chemical properties and bacterial communities in tobacco fields
[Objective]This study aims to investigate the symbiotic relationship between microalgae and Bacillus subtilis and their impact on soil bacterial communities.[Method]Four treatments,including microalgae,Bacillus subtilis,microalgae and Bacillus subtilis,and blank control,were designed.They were continuously cultured at constant temperature in room for 35 days.Soil chemical properties were determined.Using molecular biology techniques such as fluorescence quantitative PCR and Illumina Miseq sequencing,the succession rules of soil bacterial community abundance and composition were analyzed,and the effects of microalgae combined with Bacillus subtilis on soil chemical properties and bacterial community were revealed.[Result]Compared with CK treatment,microalgae combined with Bacillus subtilis significantly increased the contents of ammonium nitrogen,nitrate nitrogen,DOC and pH in soil,which increased by 11.93%,17.80%,5.58%and 2.75%respectively on the 35th day of culture.Microalgae combined with Bacillus subtilis significantly increased the richness and diversity of soil bacterial community,improved the structure of soil bacterial community,and significantly increased the relative abundance of Proteobacteria,Nitrospira and Bacillus.Redundancy analysis(RDA)showed that soil pH,available phosphorus and nitrate nitrogen content were the main environmental factors that caused the change of soil bacterial community structure.Microalgae combined with Bacillus subtilis promoted the positive interaction between soil bacterial communities,significantly increased the number of nodes,edges and average degree of network topological properties,and made the network more complex.[Conclusion]The combination of microalgae and Bacillus subtilis can effectively improve soil nutrients,regulate nutrient changes in the soil,significantly increase soil bacterial diversity,optimize soil bacterial community structure,and promote interactions between bacterial communities.
microalgaeBacillus subtilischemical properties of soilbacterial community
万方数据
维普
