Study on germination process and physiology of Lycoris radiata
[Objective]Explore the morphological and physiological changes in the germination process of Lycoris radiata seeds,and provide scientific theoretical basis and efficient reproduction technology for the breeding of high-quality seedlings.[Method]Using mature and fresh L.radiata seeds as the research material,the morphological changes were observed on days 0,7,14,21,28,and 35 during germination.Based on these observations,soluble proteins,soluble sugars and starch,endogenous hormones(GA3,ABA,IAA,ZR)were measured by coomassie brilliant blue G-250,anthrone colorimetry,enzyme-linked immunosorbent assay,respectively.[Result](1)The germination mode of L.radiata seeds is hypogeal germination,and based on morphological changes,the germination process can be divided into six stages:non-germination stage(0 d),germination stage(7-14 d),cotyledon elongation stage(8-14 d),rapid root growth stage(15-21 d),root separation stage(22-28 d),and true leaf formation stage(29-35 d).The entire germination process lasts approximately 35 days.(2)In the process of seed germination,the content of starch,soluble protein,soluble sugar,endogenous ABA and the GA3/ABA ratio generally showed a"decrease",while the content of endogenous IAA and ZR showed an"increase",while the content of GA3"decreased first and then increased".Among them,cotyledon elongation and rapid root growth period are the inflection points of endogenous hormone changes.(3)There was a very significant positive correlation between the germination process and the content changes of ZR and IAA,significant negative correlation with the content change of GA3 and ABA.This indicates that high levels of IAA and ZR,and low levels of ABA and GA3 are beneficial for the hydrolysis of starch within the endosperm,thereby promoting seed germination.[Conclusion]The germination of L.radiata seeds is a dynamic energy-consuming process,which mainly relies on the starch and soluble sugars within the endosperm to provide energy.Additionally,the germination of the seeds is positively regulated by endogenous hormones ZR and IAA,and negatively regulated by GA3/ABA.
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