首页|基于叶绿体基因片段的井冈寒竹属系统发育关系研究

基于叶绿体基因片段的井冈寒竹属系统发育关系研究

扫码查看
[目的]井冈寒竹属(Gelidocalamus Wen)属于禾本科(Poaceae)竹亚科(Bambusoideae)青篱竹族(Trib.Arundinarieae),是中国亚热带地区的特产散生竹属.该属植物为灌木状木本竹,地下茎呈复轴型,秆细长,每节多分枝,当年不再产生次级分枝,小枝末端通常仅有1片叶子,花序为圆锥型,雄蕊为3枚,秋冬季节发笋.井冈寒竹属植物主要产自中国长江以南的低海拔地区,其中南岭地区是该属植物物种多样性的分布中心,其栖息在林下、沟涧的阴凉潮湿处,呈现片状分布,具有观赏价值、食用价值和生态平衡效应.目前,该属植物的系统发育关系仍存在较大争议.本研究旨在对叶绿体基因片段的系统发育重建,初步探讨该属植物种间的亲缘关系,为深入理解竹类植物的演化历史提供一定的参考资料.[方法]试验广泛采用青篱竹族11个支系26属82种以及簕竹属(Bambusa)和牡竹属(Dendrocalamus)外类群6种作为样品.利用7个叶绿体基因片段,即rpl32-trn L、rps16-trn Q、trn C-rpo B、trn D-trn T、trn T-trn L、trn G-trn T(t)和mat K,对井冈寒竹属及其近缘类群的系统发育关系进行重建.结合通过分子钟估算,探究其起源和类群间的分化时间.[结果](1)系统发育分析结果表明,井冈寒竹属(Gelidocalamus)为复系类群,成员分别聚在青篱竹族的Shibataea clade(IV)和Phyllostachys clade(V)两个分支中;(2)分化时间估算结果表明,IV、V分支分别在5.44 Mya和3.41 Mya分化出来,除小蒙竹(G.monophyllus)外,井冈寒竹属其余种在约3 Mya间陆续分化出来.[结论]井冈寒竹属不是一个自然的单系类群;红壳寒竹(G.rutilans)应归于箬竹属下;亮竿竹(G.annulatus)可能为井冈寒竹属和箬竹属的杂交起源;冬顶寒竹(G.dongdingensis)与同春箬竹(I.tongchunensis)近缘,或为箬竹属成员,或为一独立分支.本研究通过重建井冈寒竹属的系统发育关系,对该属内成员的系统位置进行了初步阐述,为争议类群的归属问题提供了参考依据.
Phylogenetic relationships of Gelidocalamus Wen based on chloroplast gene segments
[Objective]Gelidocalamus Wen,a genus of bamboo plants,belongs to the tribe Arundinarieae in the subfamily Bambusoideae of the family Poaceae.It is a genus of scattered bamboo endemic to the subtropical regions of China.These plants are characterized by their shrubby woody stems,underground rhizomes in a complex axis,slender stems,branching at each node,with no secondary branches produced in the same year,typically one leaf at the end of each small branch,and conical flower clusters with three stamens.They also produce shoots in the autumn and winter.Gelidocalamus species are mainly found in the low-altitude south regions of the Yangtze River in China.The Nanling Region is the distribution center of species diversity of this genus.Inhabiting in the shade,cool and humid places of forests and ditches,these plants occur in patches,and they are valued for their ornamental and edible qualities as well as their ecological balance effects.However,the phylogenetic relationship of this genus is still controversial.In this study,the genetic relationship among the species of this genus was preliminarily investigated by phylogenetic reconstruction of chloroplast gene segments,which provided reference for deepening our understanding of the evolutionary history of bamboo plants.[Method]This study collected 82 species from 26 genera belonging to 11 tribes of the Arundinarieae and six species from Bambusa and Dendrocalamus as samples.Seven chloroplast gene segments,including rpl32-trn L,rps16-trn Q,trn C-rpo B,trn D-trn T,trn T-trn L,trn G-trn T(t),and mat K,were utilized to reconstruct the phylogenetic relationships of Gelidocalamus and its close relatives.Molecular clock estimation was employed to reveal the origin and divergence times between taxa.[Result]The phylogenetic analysis indicates that Gelidocalamus was a paraphyletic group,with its members clustering in two branches of the Arundinarieae tribe:the Shibataea clade(IV)and the Phyllostachys clade(V).The divergence time estimation suggests that the IV and V branches diverged around 5.44 Mya and 3.41 Mya,respectively.Apart from G.monophyllus,the other species of Gelidocalamus gradually diverged around 3 Mya.[Conclusion]Gelidocalamus is not a natural monophyletic group;G.rutilans should be classified under the genus Bambusa;G.annulatus may have originated from hybridization between Gelidocalamus and Bambusa;G.dongdingensis is closely related to I.tongchunensis and may belong to the genus Bambusa or represent an independent branch.This study performed the reconstruction of the phylogenetic relationships within Gelidocalamus,and the systematic position of the members in this genus was preliminarily expounded,which provides reference for the attribution of controversial groups.

temperate woody bambooGelidocalamus Wenphylogenetic relationshipdivergence time estimation

刘恒、汪城坤、冀雪楠、杨光耀、国春策、张文根

展开 >

江西农业大学 林学院/江西省竹子种质资源与利用重点实验室,江西 南昌 330045

温带木本竹 井冈寒竹属 系统发育关系 分化时间估算

国家自然科学基金地区基金项目国家自然科学基金地区基金项目国家自然科学基金地区基金项目

319600513196033531260043

2024

江西农业大学学报
江西农业大学

江西农业大学学报

CSTPCD北大核心
影响因子:0.748
ISSN:1000-2286
年,卷(期):2024.46(4)
  • 5