Effects of oral administration of Pseudostellaria heterophylla saponins on immune function and inflammatory response in chickens
[Objective]The aim of this study is to investigate the effects of oral administration of Radix Pseudostellariae(RP)on immune function and inflammatory response in chickens.By providing a scientific basis for the use of RP as an immunopotentiator,this study has practical application value for disease prevention and health maintenance in poultry industry.[Method]The 30-day-old healthy Hetian chickens with negative Mycoplasma gallisepticum(MG)antigen and antibody were used as subjects in the study.According to the initial body weight,they were divided into five groups,the blank group was not administered with drugs and vaccines.The vaccine control group was immunized with MG attenuated vaccine after oral administration of sterilized normal saline for 7 days.The three dose RP groups were orally administered with RP(100 mg/kg,20 mg/kg,5 mg/kg)for 7 days and then immunized with MG attenuated vaccine,the second immunization was conducted 28 days after the first immunization,and the test period was 49 days.Indirect ELISA was used to detect the growth and decline of MG-specific antibodies in serum,the positive rate of antibodies and the content of cytokines(IFN-γ,IL-4,IL-5,IL-1α,CCL4,IL-17A,IL-6).[Result](1)Compared with the vaccine control group,oral administration of RP before immunization can effectively improve the MG specific antibody titer and antibody positive rate 7 days after the first immunization and the second immunization.Among the three doses,the high dose of RP(100 mg/kg)had the best effect.(2)In the expression level of cytokines,compared with the vaccine control group,the content of immune factors(IFN-γ,IL-4,IL-5)in the serum of the RP high-dose(100 mg/kg)and medium-dose(20 mg/kg)groups increased significantly on the 3rd day after the first immunization(P<0.05),and there was no significant difference on the 7th day after the first immunization.(3)Compared with the vaccine control group,the serum levels of inflammatory cytokines(IL-1α,CCL4,IL-17A,IL-6)in the RP group were significantly decreased(P<0.05),and the high-dose group(100 mg/kg)decreased most significantly.[Conclusion]The results shows that oral administration of RP before immunization could improve the immune function of Hetian chickens and reduce the inflammatory response caused by MG attenuated vaccine,and the dose of RP 100 mg/kg has the best effect.
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