[目的]分蘖是影响水稻产量的关键因素，鉴定分蘖相关的功能基因，可为其功能的解析提供依据。[方法]以新发现的多蘖矮杆突变体high tillering and dwarf(htd5)为研究材料，鉴定突变体表型，分析已报道的多蘖矮杆功能基因在htd5中的表达量变化，并检测这些基因的编码区序列在htd5基因组中的变异情况。[结果](1)与野生型相比，突变体htd5的一次分蘖数目、二次分蘖数目以及总分蘖数目均急剧增加，而株高、一次枝梗数目、二次枝梗数目则显著降低;突变体htd5倒Ⅰ、Ⅱ、Ⅲ节间伸长受阻，各节间直径也显著缩小;此外，突变体htd5的粒宽、粒厚和千粒质量均显著降低。(2)与野生型相比，独角金内酯信号途径的正调控因子D27、D14、D3、DHT1的表达量均显著增加，而负调控因子D53的表达量显著下降;测序分析结果表明，已报道的多蘖矮杆功能基因编码区序列在htd5基因组中均没有发生变异。[结论]突变基因HTD5很可能是一个新的多蘖矮杆基因，通过调控独角金内酯信号途径，影响水稻分蘖的产生。
Biological function identification of mutant gene HTD5 regulating high tillering and dwarf traits in rice
[Objective]Tiller is a key factor affecting rice yield,and identifying genes related to tillering can lay the foundation for its functional analysis.[Method]This study used a novel high tillering and dwarf mutant(htd5)as the research material to investigate its phenotype,analyze the expression levels of high tillering and dwarf functional genes in htd5,and detect the variations in the coding region sequence of these genes in the htd5 genome.[Result](1)Compared with wild type(WT),the number of primary tiller,the number of secondary tiller,and the number of total tiller of htd5 were markedly increased,while the plant height,the number of primary and secondary branches were significantly decreased.The elongation of inversion Ⅰ,Ⅱ,and Ⅲinternodes in the mutant htd5 is hindered,and the diameter of each internode is significantly reduced.In addition,the grain width,grain thickness,and thousand grain weight of htd5 were significantly decreased.(2)Compared with WT,the expression levels of the positive regulatory factors D27,D14,D3,and DHT1 in strigolactones signaling pathway were significantly increased,while the expression levels of the negative regulatory factor D53 significantly decreased.The sequencing analysis results indicate that there is no mutation in coding region sequence of the known high tiller and dwarf genes in the htd5 genome.[Conclusion]HTD5 is likely a novel high tillering and dwarf gene that affects rice tillering by regulating strigolactones signaling pathway in rice.
万方数据
维普
