The Optimization of p-Aminobenzoic Acid Synthesis Genes Overexpression in Streptomyces
p-aminobenzoic acid(pABA,C7H7NO2),as an important nitrogen-containing precursor,can participate in the biosynthesis of secondary metabolites in actinomycetes,which is of great significance for the development of new antibiotics.To achieve accumulation of p-aminobenzoic acid in Streptomyces,the p-aminobenzoic acid synthase gene aurG and the endogenous branching acid metabolism essential gene aroAC are simultaneously(super)expressed in Streptomyces Lividans TK64 to increase the synthesis efficiency of pABA and obtain the mutant strain TK64/pABA with pABA yield of(25.063 1±0.511 8)mg·L-1.These results show that it is feasible to construct pABA biosynthesis by using functional gene combination expression,and provides a foundation for the subsequent exploration of natural drugs derived from Streptomyces.
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