The CRISPR-Cas9 type gene deletion plasmid is constructed by utilizing pWHU2653.This plasmid is used to in frame delete the core region 2 of the cluster 2,responsible for the biosynthesis of alkylresorcino in S.albovinaceus DSM 40136.Then this plasmid is finally eliminated from strain after the gene deletion.This elimination is achieved by cultivated generated mutant strains in the plates added with 5-fluorocytosine,and the ideal mutant strain S.albovinaceus DSM 40136 △ r2 is screened without a intracellular redundant plasmid.
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