首页|Streptomyces albovinaceus DSM 40136 的基因编辑改造

Streptomyces albovinaceus DSM 40136 的基因编辑改造

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该文选择基于 Clustered regularly interspaced short palindromic repeat-Cas9(CRISPR-Cas9)系统的链霉菌基因编辑质粒pWHU2653,构建特异性基因消除质粒,定位并消除在基因组上注释的alkylresorcinol生物合成基因簇的核心区段region 2,并利用质粒携带codA(sm)编码胞嘧啶脱氨酶在含有5-氟胞嘧啶的平板上进行反向筛选,获得质粒丢失的突变克隆,实现基因消除质粒的清除,构建了无痕缺失突变菌株S.albovinaceus DSM 40136 △ r2.
The Gene Editing of Streptomyces albovinaceus DSM 40136
The CRISPR-Cas9 type gene deletion plasmid is constructed by utilizing pWHU2653.This plasmid is used to in frame delete the core region 2 of the cluster 2,responsible for the biosynthesis of alkylresorcino in S.albovinaceus DSM 40136.Then this plasmid is finally eliminated from strain after the gene deletion.This elimination is achieved by cultivated generated mutant strains in the plates added with 5-fluorocytosine,and the ideal mutant strain S.albovinaceus DSM 40136 △ r2 is screened without a intracellular redundant plasmid.

Streptomycetegene editingCRISPR-Cas9gene(cluster)eliminationin frame deletion

冯杨萍、周佳鹏、刘文慧、黎金祖、黄运红、谢运昌

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江西师范大学生命科学学院,江西南昌 330022

链霉菌 基因编辑 CRISPR-Cas9 基因(簇)消除 无痕缺失

2024

江西师范大学学报(自然科学版)
江西师范大学

江西师范大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.538
ISSN:1000-5862
年,卷(期):2024.48(4)