The effect of sevoflurane pretreatment on the expression of myocardial membrane repair protein MG53 during ischemia-reperfusion
Objective The aim of this study is to explores the effect of sevoflurane pretreatment on the expression of myocar-dial membrane repair protein MG53 during ischemia-reperfusion.Methods 80 cases of interventricular septum defect requiring surgical correction were included in this study.The patients were assigned into sevoflurane pretreatment group(S-group)and control group(C-group).S-group received sevoflurane inhalation for 30 minutes after tracheal intubation,maintained at 1.0 MAC,then e-luted for 10 minutes,stop administering sevoflurane inhalation before aortic occlusion.C-group was given mixed air oxygen venti-lation.The other anesthesia intervention conditions in two groups are consistent.The concentrations of CK-MB and cTnl of the ve-nous blood in the two groups were detected at the time of anesthesia induction(T0),the time of 3 hours after arterial opening(T1),the time of 6 hours after arterial opening(T2).We collected an appropriate amount of right atrial appendage tissue of the heart at the time of beginning of CPB(T3)and at the time of hemodynamic indicators remain stable for 20 minutes after cardiac reperfusion,and used protein immunoblotting to detect the expression of MG53 in tissues.Results Respectively compared with T0,the CK MB/cTnI at T1/T2 in S-group significant increase(P＜0.05).The consistent results were also obtained in C-group.Compared with the C-group,the CK MB and cTnI in the S-group decreased at T1 and T2(P＜0.05).There was no statistically significant difference in the expression of MG53 between the two groups at T3.The expression of MG53 was lower in both groups at T4 than at the time of T3(P＜0.05).At the same time,the expression of MG53 in S-group was higher than that in C-group at T4(P＜0.05).Conclusion Preconditioning with sevoflurane can increase the expression of myocardial cell membrane repair protein MG53 during ischemia-reperfusion,which has a myocardial protective effect.
万方数据
维普
