Application value of mNGS in the detection of alveolar lavage fluid pathogens in children with lower respiratory tract in-fection
Objective This study aims to delve into the unique advantages of metagenomic next-generation sequencing(mNGS)of bronchoalveolar lavage fluid in the etiological detection of severe or refractory lower respiratory infections in children and its guiding significance for clinical treatment.Methods We retrospectively analyzed the clinical data of 50 children with se-vere or refractory lower respiratory infections admitted to the Department of Respiratory Medicine of Jiangxi Provincial Children's Hospital from October 2021 to October 2022.All patients underwent mNGS detection of bronchoalveolar lavage fluid as well as traditional methods(including sputum culture,bronchoalveolar lavage fluid culture,blood culture,serological detection of pathogens,etc.).By analyzing and interpreting the results of mNGS and traditional methods,we compared key indicators such as the positive rate,etiological distribution,monitoring time,and consistency of test results among different detection methods.Mean-while,combining the clinical data of the patients,we identified the pathogenic bacteria based on the test results and adjusted the anti-infective treatment plan accordingly.Results The positive rate of bronchoalveolar lavage fluid mNGS detection was signifi-cantly higher than that of traditional methods(96%vs 52%),and the positive rates for bacteria,viruses,and fungi were also higher than those of traditional methods.Additionally,the average time for traditional pathogen culture exceeded 72 hours,while the de-tection time for mNGS was only 24 hours,significantly reducing the detection cycle.More importantly,the guidance rate of anti-infective regimen adjustment under the guidance of mNGS was significantly higher than that of traditional methods(42%vs 6%).Conclusion The application of bronchoalveolar lavage fluid mNGS technology can significantly improve the detection rate of pathogens in severe or refractory lower respiratory infections in children,providing a rapid and accurate method for pathogen de-tection in clinical practice.At the same time,this technology can effectively guide clinical medication,improve treatment outcomes,and reduce mortality,providing a powerful new tool for the diagnosis and treatment of severe or refractory lower respiratory infec-tions in children.
Lower respiratory tract infection in childrenPathogen surveillancemNGSBronchoalveolar lavage fluid
万方数据
维普
