The expression and clinical significance of serum osteopontin in patients with rheumatoid arthritis
Objective To investigate the expression levels and clinical significance of serum osteopontin in patients with rheumatoid arthritis.Methods Sixty-eight patients with RA were selected as the RA group and sixty-three healthy patients who underwent physical examination as the control group,and the RA group was divided into bone erosion group(39 cases)and non-bone erosion group(29 cases).According to the serum anti-Cyclic citrullinated peptideantibody situation,the RA group was divid-ed into anti-CCP antibody negative group and positive group.The levels of OPN,β-CTX,DKK-1,and PINP in the serum of each group were measured by ELISA method,and bone density(including femoral neck and Ward's triangle)in the RA group was mea-sured by dual-energy X-ray absorption assay;other data were collected and compared.Results(1)The level of OPN(41.86±6.92)ng/mL,β-CTX(825.74±152.76)pg/mL,DKK-1(21.00±3.79)ng/mL in the RA group compared with the control group[(24.91±5.20)ng/mL,(482.85±103.47)pg/mL,(11.65±1.73)ng/mL]were raised,and there were statistical differences(t was15.91,15.14,18.38,respectively,P＜0.05).But the level of PINP(25.59±7.76)ng/mL in the RA group was lower than that in the control group[(47.29±7.00)ng/mL,t=-16.76,P＜0.05].(2)The level of OPN(45.14±6.45)ng/mL,β-CTX(901.65±135.31)pg/mL,DKK-1(22.38±3.57)ng/mL in the bone erosion group compared with the non-bone erosion group[(37.45±4.80)ng/mL,(723.66±110.35)ng/mL,(19.15±3.29)ng/mL]were raised,and there were statistical differences(t was 5.64,5.79,3.81,respectively,P=0.000).The level of PINP(23.68±7.44)ng/mL in the bone erosion group was lower than that in the non-bone erosion group[(28.15±7.56)ng/mL,t=-2.43,P=0.018].(3)RA anti-CCP antibody negative group OPN(45.11±7.07)ng/mL was higher than that of normal control group(24.91±5.20)ng/mL,with a strong statistical difference(t=11.00,P=0.000),but the difference was small compared with the anti-CCP positive group(40.78±6.59)ng/mL(t=2.30,P=0.020).(4)Bone bone density(including femur neck and Ward's triangle)was lower in the bone erosion group[(0.61±0.11)g/cm2 vs(0.67±0.09)g/cm2,t=2.28,P=0.030;(0.43±0.12)g/cm2 vs(0.54±0.13)g/cm2,t=3.76,P=0.000].(5)Correlation analysis showed that OPN was positively correlated with DAS28(ESR),β-CTX,and DKK-1(r was 0.30,0.40,0.37,P＜0.05),was negatively correlated with PINP(r=-0.31,P＜0.05),and had no correlation with anti-CCP antibodies(r=-0.06,P=0.640).(6)ROC curve analysis suggests that serum OPN has good predictive value for both RA bone erosion and disease activity.Conclusion The serum OPN levels in patients with RA were significantly higher than in the healthy group and more pronounced in the bone erosion group.OPN was positively correlated with bone resorption and RA disease activity,suggesting that OPN may be involved in the RA bone erosion process and promote RA disease activity.The serum OPN is elevated in the anti-CCP-nega-tive group,making OPN another biomarker for diagnosing RA.
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