Effect of Yupingfeng on T lymphocyte subsets and related cytokines in a mouse model of asthma
Objective This study aims to investigate the effects of Yupingfeng,a traditional Chinese medicine,on T lympho-cyte subsets and related cytokines in asthmatic mice,to evaluate its potential therapeutic benefits for asthma.Methods The ex-periment involved 24 female SPF-grade BALB/c mice,randomly divided into three groups:control,asthma model,and Yup-ingfeng-treated.Asthma was induced in the asthma model and Yupingfeng groups using ovalbumin(OVA)sensitization and chal-lenge.The Yupingfeng group received daily oral administration of Yupingfeng granules.The lung tissues were analyzed using PAS staining to observe epithelial goblet cell proliferation and mucus secretion.The levels of cytokines(IL-4,IL-5,IL-13,IL-10,IFN-γ,and IL-17)in bronchoalveolar lavage fluid were measured using ELISA.Flow cytometry was used to assess the proportions of T lymphocyte subsets(Th1,Th2,Th17,Treg)in peripheral blood.Results Yupingfeng treatment significantly reduced airway hyper-responsiveness and inflammation in asthmatic mice.PAS staining showed reduced goblet cell proliferation and mucus secretion in the Yupingfeng group compared to the asthma model group.ELISA results indicated that the Yupingfeng-treated group had signifi-cantly lower levels of pro-inflammatory cytokines(IL-4,IL-5,IL-13,IL-17)and higher levels of anti-inflammatory cytoki nes(IL-10,IFN-γ)compared to the asthma model group.Flow cytometry revealed that Yupingfeng normalized the Th1/Th2 balance,re-ducing Th2 and Th17 cells while increasing Th1 and Treg cells.Conclusion Yupingfeng demonstrates significant immunomodula-tory effects in asthmatic mice by restoring the balance of T lymphocyte subsets and modulating cytokine production.These findings suggest that Yupingfeng could be a potential alternative or adjunctive treatment for asthma,warranting further clinical investigation.
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