Objective Glioblastoma(GBM)is one of the most deadly diseases that seriously endanger human health all over the world.Xuefu Zhuyu Decoction(XFZYD)is a prescription for promoting blood circulation and removing blood stasis.Although XFZYD has been proved to inhibit the migration and invasion of tumor cells,its active components,potential targets and potential mechanisms are still unclear.The purpose of this study is to explore the molecular mechanism of XFZYD in treating GBM through network pharmacology.Methods GBM gene expression matrix was downloaded from GEO database,and the active components and targets of XFZYD were screened by TCMSP database.Further screening the active compounds by absorption,distribution,metabolism and excretion(ADME)characteristics;Take the intersection(common target)between the target of XFZYD active ingre-dient and the differentially expressed gene of GBM to obtain the potential target of XFZYD in treating GBM;The"active ingredi-ent-target"network of XFZYD was constructed by Cytoscape software.Protein-protein interaction(PPI)network analysis and func-tional enrichment analysis were performed on the common targets.Results We identified 117 active components by absorption,distribution,metabolism and excretion(ADME)screening,and identified 2265 differential genes by differential expression analysis,including 1 192 genes with up-regulation and 1 073 genes with down-regulation.The component-target-pathogenic gene(C-T-P)network integrated by component-target network and GBM-related genes shows that XFZYD can treat GBM through 72 related dif-ferential genes,and then the component-target-pathogenic gene network(C-T-P network)is analyzed by CytoNCA plug-ins and MCODE plug-ins in Cytoscape.The results suggest that EGFR,BCL2-2 and FOS are the core genes.Functional analysis suggests that XFZYD mainly treats GBM through PI3K-Akt signaling pathway.Conclusion XFZYD has the characteristics of multi-com-ponent,multi-target and multi-channel,which is mainly related to PI3K-Akt signal pathway.
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