Objective To establish a rat model of necrotizing enterocolitis(NEC)and study the inhibitory effect of Astragalus polysaccharide(APS)intervention on intestinal inflammatory response.Methods NEC was induced in neonatal rats using artificial milk substitutes,hypoxia,and cold stress.The APS+NEC intervention group was given APS intervention in advance,and the con-trol group and APS group were fed with common milk powder.The pathological changes of intestinal tissue were analyzed by hematoxylin-eosin(HE)staining.Apoptosis was detected by TdT-mediated dUTP Nick-End Labeling(TUNEL).The expression levels of TNF-α and IL-6 in intestinal tissues were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of apoptosis and inflammation-related proteins(C-Caspase-3,BAX-,IL-1 β and iNOS)were analyzed by immunohistochemical staining.Results The intestinal pathological injury score of neonatal rats in NEC group was significantly higher than that of control and APS groups,and APS intervention can significantly reduce the pathological injury scores(P＜0.05);The levels of the inflamma-tory factors TNF-α,IL-6 and the proportion of apoptotic cells in the intestinal tissues were decreased in APS+NEC intervention group compared with the NEC group(P＜0.05,P＜0.001);The immunohistochemistry results showed that the expression levels of pro-apoptotic proteins(C-Caspase3,BAX)and inflammation-related proteins(IL-1β,iNOS)in APS+NEC intervention group were sig-nificantly reduced than those in the NEC group.Conclusion APS significantly alleviate intestinal tissue damage in NEC rat mod-els by inhibiting the expression of intestinal inflammatory factors and related apoptotic proteins.
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