Isolation and identification of the pathogen causing rice leaf stripes in Daoxian County of Hunan Province
[Objective]In June 2023,a new leaf stripe disease was discovered on rice in some villages of Xianzijiao Town,Daoxian County,Yongzhou City,Hunan Province,with an area of over 30 hm2.To investigate the cause of the disease,relevant researches were conducted.[Method]The pathogen was isolated from diseased rice leaves collected from Daoxian County by using the tissue isolation method.Morphological observation combined with ITS,TEF1-α and TUB2 gene sequence evolutionary tree were used to identify the pathogen,and then the pathogen was validated by the Koch's rule.[Result]The pathogen of the rice streak leaf spots was identified as Nigrospora oryzae.[Conclusion]The pathogen of the rice leaf stripe disease was identified,providing references for the prevention and control of the disease in the future.
rice leaf stripe diseaseisolation and identificationKoch's ruleNigrospora oryzae
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