Preparation of Cu-Fe bimetallic nanoparticles and their activity against oomycetes and fungi
[Objective]The emergence of metal nanomaterials has demonstrated excellent efficiency,and the application of new metal nanomaterials for antibacterial purposes in agriculture still needs to be explored.[Method]In this study,the chemical reduction method was used to prepare Cu-Fe NPs with excellent antifungal activities.The mycelial growth rate method was employed to study the Cu-Fe NPs against several common plant pathogenic microorganisms.[Result]The study showed that the mixed solution of 10 mL CuSO4 and FeCl3 was dropped into 30 mL catechol solution(0.001 mol/L)with pH of 11 at a rate of 4 mL/min,and the ultrasonic reaction lasted for 30 min,which could obtain Cu-Fe NPs with irregular morphology,uniform particle size distribution and an average particle size of 30 nm.Cu-Fe NPs could significantly inhibit the mycelial growth of Phytophthora melonis,Alternaria solani,Valsa mali and Alternaria aphanin with EC50 values of 325.05,334.00,429.64 and 325.62 mg/L,respectively.[Conclution]Cu-Fe NPs have good potential to inhibit the growth of pathogenic microorganisms,and the research results can provide data support for the prevention and treatment of fungal diseases with metal nanoparticles,and provide references for the application of nano-pesticides.
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