首页|江西鄱阳湖候鸟沙门氏菌的分离鉴定与耐药性分析

江西鄱阳湖候鸟沙门氏菌的分离鉴定与耐药性分析

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[目的]为研究候鸟沙门氏菌对常见抗菌药物的耐药性。[方法]通过对江西省鄱阳湖采集的 108 份候鸟粪便样品进行处理,使用平板划线法进行细菌分离和菌种鉴定,通过纸片扩散法对菌株进行 8 种常用抗菌药物的敏感性检测,统计数据后进行药物敏感性、多重耐药和耐药谱分析。[结果]共分离到 96 株沙门氏菌,阳性率为 88。89%;对 8 种常用抗菌药物表现出不同程度的耐药,耐药率为 88。54%;其中对氨苄西林的耐药率最高(64。58%),其次是庆大霉素(42。71%)、链霉素(39。58%)、四环素(33。33%)、环丙沙星(23。96%)、头孢噻肟(17。71%)、诺氟沙星(8。33%)、氯霉素(4。17%);对诺氟沙星敏感性最高(61。46%),其次是氯霉素(60。42%)、链霉素(37。50%)、庆大霉素(36。46%)、环丙沙星(36。46%)、四环素(20。83%)、头孢噻肟(13。54%)氨苄西林(8。33%);分离的 96 株鸟源沙门氏菌共产生 39 种耐药谱组合,存在 6 重耐药谱,其中以 1~4 重耐药为主,多重耐药率达 42。71%。[结论]该地区的沙门氏菌对抗菌药物产生耐药,出现多重耐药且其耐药谱呈现多样性。
Isolation,identification and antimicrobial drug resistance analysis of Salmonella from migratory birds at Poyang Lake,Jiangxi Province
[Objective]This study was to discuss the resistance analysis of migratory bird-derived Salmonella to common antimicrobial drugs.[Method]In this experiment,108 fecal samples of migratory birds collected from Poyang Lake,Jiangxi Province,were processed for bacterial isolation by using the plate delineation method and strain identification,and the strains were tested for susceptibility to eight commonly used antimicrobial drugs by the paper diffusion method.Then the statistical data were analyzed for drug susceptibility,multi-drug resistance and drug resistance spectrum.[Result]The results showed that 96 strains of Salmonella were isolated,of which the positivity rate was 88.89%;Salmonella showed different degrees of resistance to 8 commonly used antimicrobial drugs,and the resistance rate was 88.54%;the drug resistance rate to ampicillin(64.58%)was the highest,followed by the gentamicin(42.71%),streptomycin(39.58%),tetracycline(33.33%),ciprofloxacin(23.96%),cefotaxime(17.71%),norfloxacin(8.33%),and chloramphenicol(4.17%);the Salmonella were highly sensitive to norfloxacin(61.46%),followed by the chloramphenicol(60.42%),streptomycin(37.50%),gentamicin(36.46%),ciprofloxacin(36.46%),tetracycline(20.83%),cefotaxime(13.54%),and ampicillin(8.33%);39 drug resistance spectrum combinations were produced in 96 strains of Salmonella of avian origin isolated,all of which were more than 6 times,mainly 1~4 times,and the rate of multi-drug resistance reached 42.71%.[Conclusion]Salmonella in this region is resistant to antimicrobial drugs,showing multi-drug resistance and diversity in its drug resistance spectrum.

Salmonellaisolation and identificationdrug resistance analysisdrug resistance spectrum

梁惠平、黄鹏、严珲、邓海欣、曹华斌、吴欢生

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江西农业大学 动物科学技术学院/江西省畜禽疾病诊断与防治重点实验室,江西 南昌 330038

江西省野生动植物保护中心,江西 南昌 330006

沙门氏菌 分离鉴定 耐药分析 耐药谱

2024

生物灾害科学
江西农业大学江西省昆虫学会江西省植物保护学会江西省植物病理学会

生物灾害科学

影响因子:0.5
ISSN:2095-3704
年,卷(期):2024.47(4)