首页|间型脉孢菌JY-05211固态发酵玉米秸秆条件优化

间型脉孢菌JY-05211固态发酵玉米秸秆条件优化

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为探究间型脉孢菌JY-05211对秸秆中粗纤维的降解能力,以玉米秸秆为发酵原料,以JY-05211为发酵菌种进行固体发酵,通过单因素及正交试验对发酵培养基组分和培养条件进行优化.结果表明,玉米秸秆固体发酵最适培养基配方为玉米秸秆98.45%、蛋白胨1.5%、氯化钙0.05%;最适培养条件为:料液比1∶2.1、培养温度28 ℃、发酵时间7d,在此条件下发酵玉米秸秆其粗纤维降解率为36.58%.研究为玉米秸秆等的高值化利用提供了思路和方法.
Optimization of Solid State Fermentation Conditions for Corn Stalks by Neurospora Intermedia
To investigate the degradation ability of crude fiber in stalk by Neurospora intermedia JY-05211,the selection and optimization of medium constitutes as well as culture conditions was carried out with corn stalks as main material and JY-05211 as fermentaiton strain using one-factor-at-a-time method combined with orthogonal experiment.As a result,the optimal levels for corn stalks,peptone,calcium chloride,material liquid ratio,culture temperature and culture time were determined as 98.45%,1.5%,0.05%,1∶2.1,28 ℃ and 7 d,respectively.Confimatory experiments performed under this conditions yielded a maximam of crude fiber degradation rate 36.58%.This study provides a thought and method for the high value utilization of corn stalks.

neurospora intermediasolid state fermentationcorn stalkorthogonal optimization

付文娟、汪启源、朱英卓、高仕、赖幼纯、李绪锐、陈韵琪、袁景欣、李红梅、李月婷、王庆

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广东省山区特色农业资源保护与精准利用重点实验室,广东梅州 514015

梅州市生物饲料工程技术研究中心,广东梅州 514015

嘉应学院生命科学学院,广东梅州 514015

广东代米生物科技有限公司,广东梅州 514300

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间型脉孢菌 固体发酵 玉米秸秆 正交优化

2024

嘉应学院学报
嘉应学院

嘉应学院学报

CHSSCD
影响因子:0.25
ISSN:1006-642X
年,卷(期):2024.42(6)