摘要
为探究间型脉孢菌JY-05211对秸秆中粗纤维的降解能力,以玉米秸秆为发酵原料,以JY-05211为发酵菌种进行固体发酵,通过单因素及正交试验对发酵培养基组分和培养条件进行优化.结果表明,玉米秸秆固体发酵最适培养基配方为玉米秸秆98.45％、蛋白胨1.5％、氯化钙0.05％;最适培养条件为:料液比1∶2.1、培养温度28 ℃、发酵时间7d,在此条件下发酵玉米秸秆其粗纤维降解率为36.58％.研究为玉米秸秆等的高值化利用提供了思路和方法.
Abstract
To investigate the degradation ability of crude fiber in stalk by Neurospora intermedia JY-05211,the selection and optimization of medium constitutes as well as culture conditions was carried out with corn stalks as main material and JY-05211 as fermentaiton strain using one-factor-at-a-time method combined with orthogonal experiment.As a result,the optimal levels for corn stalks,peptone,calcium chloride,material liquid ratio,culture temperature and culture time were determined as 98.45％,1.5％,0.05％,1∶2.1,28 ℃ and 7 d,respectively.Confimatory experiments performed under this conditions yielded a maximam of crude fiber degradation rate 36.58％.This study provides a thought and method for the high value utilization of corn stalks.
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