Determination of several novel synthetic cannabinoids in different food matrices by ultra-high performance liquid chromatography-high resolution mass spectrometry
Determination of several novel synthetic cannabinoids in different food matrices by ultra-high performance liquid chromatography-high resolution mass spectrometry
A ultra-high performance liquid chromatography-high resolution mass spectrometry method was developed to simul-taneously determine the levels of natural cannabinoids and synthetic cannabinoid compounds in various food samples.Addi-tionally,a high-resolution mass spectrometry was utilized for the analysis of characteristic fragment ions of cannabinoid com-pounds,leading to the establishment of a secondary spectral library.The samples were extracted with methyl alcohol,purified using HLB solid-phase extraction column,separated by C18 chromatographic column,and eluted with a gradient of methanol and 2 mmol ammonium acetate solution containing 0.1％formic acid.The analysis was performed by external standard method using electrospray ionization in positive ion mode,employing multiple reaction monitoring mode for detection,and matrix matching was employed to eliminate matrix effects.The developed method exhibited excellent linearity with correlation coefficients(R2)greater than 0.999 across the concentration range of 1-500 μg/L.The limit of detection(LOD)for cannabi-noid compounds in biscuits and cakes was determined to be 3 pg/kg,with a limit of quantification(LOQ)of 10 μg/kg.In beverages,the LOD for cannabinoid compounds was found to be 0.6 μg/kg,with a LOQ of 2 μg/kg.The three-level spiked recovery of cannabinoid compounds in three different food matrices,namely biscuits,cakes,and beverages,yielded average re-covery rates ranging from 75.1％to 106.7％.The relative standard deviation(RSD)ranged from 2.5％to 10.3％(n=6),indi-cating excellent precision and sensitivity of the method.These results demonstrate that the method is capable of simultane-ous determination of cannabinoid compounds in commonly encountered food matrices,and the utilization of the secondary spectral library enables effective identification of these compounds.
关键词
新型合成大麻素/超高效液相色谱/高分辨质谱/食品
Key words
Synthetic cannabinoids/Ultra-high performance liquid chromatography/High resolution mass spectrometry/Food
维普
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