Determination of chloramphenicol residues in silkworm excrement-high performance liquid chromatography-tandem mass spectrometry method with QuEChERS
A method for the determination of chloramphenicol residues in silkworm excrement based on QuEChERS-High Performance liquid chromatography-tandem mass spectrometry was developed.The method was extracted by ethyl acetate,pu-rified by GCB and C18 solid phase extraction column,eluted by methanol and water as mobile phase gradient,separated by C18 column(2.1 mm×100mm,1.7 μm),determined by liquid chromatography tandem mass spectrometry under the negative ion mode of electric spray ion source(ESI),and quantified by internal standard method.The results showed that chloramphenicol had a good linear relationship between 0.02 μg/L-5.00 μg/L with a correlation coefficient of 1.000.The recoveries of chlo-ramphenicol at four supplemental levels(0.1 μg/kg,0.3 μg/kg,1.0 μg/kg,2.0 μg/kg)ranged from 74.7％-103.0％,and the RSD ranged from 4.36％-9.64％.The method is quick,simple,sensitive and has high recovery rate,so it can be used for the de-tection and analysis of chloramphenicol residues in silkworm excrement.
Silkworm excrementChloramphenicolQuEChERSSolid phase extractionHigh performance liquid chromatogra-phy-tandem mass spectrometry
万方数据
维普
