Determination of lutein in natural color cocoons by high performance liquid chromatography
The lutein in natural color cocoons was extracted by ultrasonic and detected by high performance liquid chromatography,the chromatography was performed on ZORBAZ Extend C18(4.6×150 mm,5 μm)with mobile phase consisting of methanol∶water(95∶5),the flow rate was 1 mL/min,the detection wavelength was 450 nm.The results showed that the lutein concentration exhibits linearity within the range of 0.5 μg/mL to 10 μg/mL,with a correlation coefficient R2 of 0.999,the recovery was 96%-100%,and the relative standard deviation was 0.33%,it is thus clear that the method could be used for the determination of lutein in natural color cocoons.
NETL
NSTL
万方数据
