Establishment of detection of nucleic acid in Staphylococcus aureus by fluorescent probe
To establish a rapid,sensitive fluorescent probe polymerase chain reaction(PCR)method for the detection of Staphylococcus aureus.Specific primers and probes were designed for the Staphylococcus aureus nuc gene,and the plasmid standard and pure culture standard were prepared,and the method was established.Then,the specificity,sensitivity,repeatabili-ty and practicability of the method were evaluated.As a result,the specificity of this method was 100％,the sensitivity of plasmid was 1 copy/μL,the sensitivity of pure culture level was 1 000 CFU/mL,the coefficient of variation of repeatability was 0.69％,various matrix spiked samples were effectively detected.A fast and sensitive method for nucleic acid detection has been successfully established,which can be used for rapid nucleic acid detection of suspected Staphylococcus aureus contam-inated samples.
万方数据
维普
