首页|2022年昆明市学生餐中微生物污染现状分析及其菌种鉴定

2022年昆明市学生餐中微生物污染现状分析及其菌种鉴定

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为了解2022年昆明市学生餐中微生物污染现状,按照国家食品安全标准对昆明市的学生餐开展监测,利用统计软件SPSS 27.0对监测结果进行数据统计分析,采用RiboPrinter®全自动微生物基因指纹鉴定系统对检出的污染菌进一步鉴定分析.结果显示,对36家集体用餐配送企业的385批次盒饭开展检验,不合格26批次,总体不合格率为6.8%,其中,菌落总数不合格率4.2%,大肠埃希氏菌不合格率3.4%,金黄色葡萄球菌、沙门氏菌全部合格.污染菌纯化分离后得到68株菌株,进一步鉴定出51株菌株,包括19种细菌属,主要为芽孢杆菌属23株,占比33.8%;葡萄球菌属6株,占比8.8%;肺炎克雷伯氏菌5株,占比7.3%;大肠埃希氏菌属6株,占比8.8%;少动鞘氨醇单胞菌3株,占比4.4%;克氏库克菌、藤黄微球菌各2株,占比2.9%;不动杆菌、黄色氢噬胞菌、阴沟肠杆菌、嗜麦芽窄食单胞菌各1株,占比1.5%.由此可见,2022年昆明市学生餐食品安全总体状况良好,未发现高风险致病菌,但集体用餐配送企业应对条件致病菌引起重视,必须严格控制卫生条件,预防学生群体食源性疾病的发生.
Analysis of the current situation of microbial pollution in Kunming students in 2022 and the identification of its strains
In order to understand the current situation of microbial pollution in students'meals in Kunming in 2022,the stu-dents'meals in Kunming were monitored and analyzed according to the national food safety standards.The statistical soft-ware SPSS 27.0 was used to analyze the monitoring results,and RiboPrinter® automatic microbial gene fingerprint identifi-cation system was used to further identify and analyze the contaminated bacteria.The results showed that 385 batches of box lunch from 36 group meal distribution enterprises were inspected,and 26 batches were unqualified,and the overall un-qualified rate was 6.8%.Among them,the unqualified rate of the total number of bacterial colonies was 4.2%,the unquali-fied rate of Escherichia coli was 3.4%,and Staphylococcus aureus and salmonella were all qualified.Contaminating bacteria were purified and isolated from 68 strains,51 strains were further identified,including 19 species of bacterial genera,Mainly for 23 strains of the genus Bacillus species,33.8%;Six strains of the Staphylococcus genus,8.8%;Five strains of Klebsiella pneumoniae,accounting for 7.3%;Six strains of Escherichia coli,8.8%;Three strains of Sphingomonas paucimobilis oligolig-otile spphingomonas,4.4%;F.kerneri and Micrococcus 2 strains each,2.9%;Acinetobacter,xanosis,Enterobacter cloacae,Stenotrophomonas maltophilia,accounting for 1.5%.It can be seen that in 2022,the overall food safety of students in Kun-ming is good,and no high-risk pathogenic bacteria were found,but the group meal distribution enterprises should pay atten-tion to the conditional pathogenic bacteria,and must strictly control the sanitary conditions to prevent the occurrence of food-borne diseases among students.

Student mealsBacterial contaminationRiboPrinter® automatic microbial gene fingerprint identification systemIden-tification and analysisMicrobial community

张加稳、赵四标、刁玉华、张联琴、刘羽晏、左诚、王蕊、李应才

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昆明市食品药品检验所 云南 昆明 650034

学生餐 细菌污染 RiboPrinter®全自动微生物基因指纹鉴定系统 鉴定分析 微生物群落

2024

质量安全与检验检测
中国检验检疫科学研究院

质量安全与检验检测

影响因子:0.399
ISSN:2096-8876
年,卷(期):2024.34(5)