Result analysis and uncertainty evaluation of capability verification for mold and yeast
Verify the laboratory's inspection capabilities and optimize data processing capabilities by participating in competency verification.The test was carried out according to GB 4789.15-2016.The accuracy of data is improved by increasing the number of parallel samples and strictly controlling the counting time.According to JJF 1059.1-2012,the uncertainty was evaluated from dilution ratio(sample stock solution preparation,incremental dilution),adding volume and counting results.The test result for 23-I389 was 1 000 CFU/mL and the test result for 23-F825 was 190 CFU/mL.The value of|Z|of the two capability verification samples is lower than 2.0,and the result is satisfactory.The uncertainty of sample 23-I389 is 3.50×10-2 and that of sample 23-F825 is 3.20×10-2.The counting time should be strictly controlled after 24 hours to prevent the spread of colonies from affecting the count.The standard uncertainty by the dilution of a single sample is the largest proportion,so the laboratory temperature should be strictly controlled and the measurement should be accurate during the experiment.The testing ability of mold and yeast count in this laboratory can meet the requirements.Data processing capacity has been significantly improved.