Determination of 36 anti-infective drugs in toothpaste by UPLC
A highly efficient liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was established for the simultaneous determination of 36 anti-infective drugs in toothpaste.Samples were first dispersed with saturated sodium chlo-ride solution and then extracted with acetonitrile using ultrasonication.Separation was achieved through the Phenomenex Kinetex C18 chromatographic column(50×3.0 mm,2.6 pm),with gradient elution using ammonium acetate solution and ace-tonitrile.Ionization was performed in both positive and negative ion modes using an electrospray ionization(ESI)source,and the compounds were detected using multiple reaction monitoring(MRM)mode.The results demonstrated that the 36 an-ti-infective drugs had good linear relationships within the range of 50-800 ng/mL,with correlation coeffiicients greater than 0.99.The limits of detection(LOD)ranged from 0.003 to 0.250 µg/g,with average recoveries between 78.1％-119.2％,and rel-ative standard deviations(RSD)ranging from 0.6％-8.5％.When the method was applied to test 20 batches of commercially available toothpaste samples,none of these anti-infective drugs were detected.The method exhibits good sensitivity,precision and accuracy,making it suitable for the determination of anti-infective drugs in toothpaste.
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