Analysis of the reasons for contamination in the upright cultivation method about GB 4789.15-2016 and corresponding solutions
Compared with the previous edition,the most significant alteration in the first method of GB 4789.15-2016 is the shift from the inverted culture method to the upright culture method,but the upright culture method sometimes leads to the contamination of the blank control.In this paper,experiments were designed based on the five elements of"human,machine,material,method and environment",found that the contamination of the blank control is mainly attributed to the air flow caused by the blower system inside the incubator,and through the wind speed increase test,the cause was confirmed.The contamination issue of the upright culture method can be resolved through the addition of a breathable protective bag test.
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