奶粉中蜡样芽胞杆菌检测能力验证结果和关键控制点分析
Analysis on the verification results of detection ability and key control point of Bacillus cereus in milk powder
余秋地 1秦爱 1张明娟 1袁磊 1刘明明 1王娟1
作者信息
- 1. 重庆市计量质量检测研究院 重庆 401121
- 折叠
摘要
为提升微生物实验室蜡样芽胞杆菌检测能力,加强实验室质量控制,实验室参加了国家市场监督管理总局食品安全抽检监测司组织的"奶粉中蜡样芽胞杆菌检验能力评价".根据作业指导书和GB 4789.14-2014《食品安全国家标准食品微生物学检验蜡样芽胞杆菌检验》对样品编号为NFLY-43和NFLY-82的2份能力验证样品进行定量检测,并对检测过程和结果进行关键控制点分析.综合革兰氏染色镜检、VITEK 2 Compact鉴定、根状生长试验和蛋白质毒素结晶试验等生化鉴定结果,样品NFLY-43结果报告为1.1×104CFU/g,样品NFLY-82结果报告为6.2×104CFU/g,其检测结果获得满意评价.
Abstract
In order to enhance the detection capability of Bacillus cereus in the microbiology laboratory and strengthen labo-ratory quality control,the laboratory participated in the Evaluation on the Inspection Ability of Bacillus cereus in Milk Pow-der organized by the Food Safety Sampling and Monitoring Department of the State Administration of Market Supervision and Administration.According to the requirements of the operation instructions and GB 4789.14-2014 National Food Safety Standard for Microbiological Examination of Food-Bacillus cereus,quantitative testing was conducted on two proficiency testing samples with sample numbers NFLY-43 and NFLY-82,and key control points of the test process and results are ana-lyzed..Based on the biochemical identification results of Gram staining microscopy,VITEK 2 Compact identifiication,root growth tests and protein toxin crystallization tests,the results of NFLY-43 and NFLY-82 were reported to be 1.1×104CFU/g and 6.2×104 CFU/g,and the results were satisfactory.
关键词
蜡样芽胞杆菌/能力验证/质量控制Key words
Bacillus cereus/Capability verification/Quality control引用本文复制引用
出版年
2024
NETL
NSTL
万方数据