Objective To explore the effect of active vitamin D on autophagy,inflammation and apoptosis of nucleus pulposus cells in high glucose environment.Methods In vitro,human nucleus pulposus cells(NPCs)were cultured in normal glucose(nor-mal glucose group)and high glucose(high glucose group)environment,and the autophagy,inflammation and apoptosis of NPCS were detected for the first time.Active vitamin D was added in two groups,and the autophagy,inflammation and apoptosis of NPCs were de-tected again after continued culture.The factors of IL-1β,TNF-α and H2O2 were detected by ELISA,SOD,Caspase 3,Atg5,Atg12 and LC3 were detected by Western blotting(WB).Results Compared with normal glucose group,autophagy of NPCs decreased,ox-idative stress and apoptosis increased in high glucose group,with statistical significance(P＜0.05).There was no statistically signifi-cant difference in the change of biological characteristics of NPCs in the normal glucose group(P＞0.05),while the low autophagy of NPCs was improved and enhanced,the oxidative stress and inflammatory response was weakened,and the apoptosis decreased in the high glucose group after adding active vitamin D,with statistical significance(P＜0.05).Conclusions Active vitamin D protects NPCs and delays disc degeneration by activating autophagy and inhibiting inflammation and apoptosis in high glucose environment.
维普
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