microRNA-132及其靶基因NAG-1在评估强直性脊柱炎患者非甾体类抗炎药治疗效果中的预测价值

Predictive value of NSAIDs in the treatment of ankylosing spondylitis

张连锁 ¹蔡金生 ¹王会旺 ¹孙亚东 ¹宁胜华 ¹徐伟坤¹

扫码查看

作者信息

1. 华北医疗健康集团邢台总医院骨六科,河北邢台 054000
折叠

摘要

目的探讨microRNA-132及其靶基因NAG-1在评估强直性脊柱炎(AS)患者非甾体类抗炎药治疗效果中的预测价值.方法选择在该院就诊的AS患者154例作为观察组,抽取同期在该院体检的健康受试者154例作为对照组.观察两组受试者miR-132和NAG-1 mRNA表达水平.观察组患者采用双氯芬酸钠治疗16周,根据美国风湿病学协会(ACR)制定的ACR20标准,将观察组达到标准患者分为ACR20组,其余患者归为非ACR20组,比较两组治疗前miR-132和NAG-1mRNA表达水平及其对疗效的预测价值.结果观察组患者单核细胞miR-132 mRNA表达水平高于对照组,NAG-1 mRNA表达水平低于对照组,差异均有统计学意义(P＜0.05).miR-132预测AS的灵敏度为83.8％,特异性为98.1％,最佳截断值为1.373,曲线下面积(AUC)为0.964,95％CI为0.936～0.982;NAG-1预测AS的灵敏度为76.0％,特异度为86.4％,最佳截断值为0.826,AUC 为 0.879,95％CI 为 0.838～0.914.ACR20 组患者 miR-132mRNA 表达水平低于非 ACR20 组患者,NAG-1mRNA表达水平高于非ACR20组患者,差异均有统计学意义(P＜0.05).miR-132预测ACR20的敏感度为81.8％,特异性为94.2％,AUC 为 0.950,95％CI 为 0.903～0.979;NAG-1 预测 ACR20 的敏感度为 97.0％,特异性为 46.3％,AUC 为 0.751,95％CI为0.675～0.817.结论 miR-132及NAG-1可能是诊断AS及预测非甾体类抗炎药治疗效果的潜在生物学指标.

Abstract

Objective To investigate the predictive value of microRNA-132 and its target gene NAG-1 in evaluating the therapeu-tic effect of nonsteroidal anti-inflammatory drugs in patients with ankylosing spondylitis(AS).Methods 154 patients with AS in oui hospital were selected as the observation group,and 154 healthy subjects who underwent physical examination in our hospital in the same period were selected as the control group.The expression levels of miR-132 and NAG-1 mRNA in the two groups were observed.Patients in the observation group were treated with diclofenac sodium.The expression levels of miR-132 and NAG-1 mRNA before treatment were observed to predict the efficacy of American Rheumatology Association(ACR)20 after treatment.Results The ex-pression level of miR-132 mRNA in mononuclear of patients in the observation group was higher than that in the control group,and the expression level of NAG-1 mRNA was lower than that in the control group(P＜0.05).The sensitivity of miR-132 in predicting AS was 83.8％,the specificity was 98.1％,the best cut-off value was 1.373,the area under the curve(AUC)was 0.964,and the 95％CI was 0.936-0.982.The sensitivity of NAG-1 in predicting AS was 76.0％,the specificity was 86.4％,the best cut-off value was 0.826,AUC was 0.879,and 95％CI was 0.838-0.914.The expression level of miR-132 mRNA in ACR20 group was lower than that in non ACR20 group,and the expression level of NAG-1 mRNA was higher than that in non ACR20 group(P＜0.05).The sensitivity of miR-132 in predicting ACR20 was 81.8％,the specificity was 94.2％,the AUC was 0.950,and the 95％CI was 0.903-0.979.The sensitivity of NAG-1 to predict ACR20 was 97,0％,the specificity was 46.3％,the AUC was 0.751,and the 95％CI was 0.675-0.817.Conclusions miR-132 and NAG-1 may be potential biological indicators of the therapeutic effect of AS and non steroidal anti-inflammatory drugs.

关键词

微小RNA/非甾体类抗炎药激活基因/强直性脊柱炎/非甾体类抗炎药

Key words

microRNA/nonsteroidal anti-inflammatory drug activating gene/ankylosing spondylitis/non steroidal anti-inflamma-tory drugs

引用本文复制引用

出版年

2024

颈腰痛杂志

安徽医科大学

颈腰痛杂志

CSTPCD

影响因子：1.006

ISSN：1005-7234

段落导航