Effect of ginsenoside Rg3 regulating RANKL/RANK/TRAF6 signaling pathway on bone metabolism and osteoblast in postmenopausal osteoporosis rats
Objective To analyze the effect of ginsenoside Rg3 on regulating and nuclear factor-κB receptor activator ligand(RANKL)/nuclear factor-aB receptor activator(RANK)/tumor necrosis factor receptor-re-lated factor 6(TRAF6)signaling pathway on bone metabolism and osteoblast in postmenopausal osteoporosis rats.Methods Fifty healthy female SPF SD rats were selected as experimental objects,and 10 rats were ran-domly selected as control group,and the remaining 40 rats were established for postmenopausal osteoporosis model,of which 30 rats were successfully modelled,and 30 rats were randomly divided into model group,gin-senoside Rg3 low dose group and ginsenoside Rg3 high dose group,with 10 rats each.The pathological tissue of rats in each group was observed,and the levels of bone metabolism,bone morphology,alkaline phosphatase(ALP),parathyroid hormone(PTH),osteocalcin and osteosin,as well as messenger RNA(mRNA)and protein levels related to RANKL/RANK/TRAF6 signaling pathway were compared among all groups.Results In the control group,the bone trabecular arrangement was normal,and the area of osteoblasts and osteoid did not change,and the number of osteoblasts decreased in the model group.Compared with the model group,the number of osteoblasts in ginsenoside Rg3 low dose group and ginsenoside Rg3 high dose group increased,and the number of osteoblasts in ginsenoside Rg3 high dose group was more than that in ginsenoside Rg3 low dose group.Tra-becular quantity(TB.N),trabecular thickness(TB.Th),total type Ⅰ collagen carboxyl terminal propeptide(PⅠ NP),N-terminal osteocalcin(N-MID),PTH,osteocalcin,osteosin,RANKL mRNA,RANK mRNA,TRAF6 mRNA,RANKL protein,RANK protein and TRAF6 protein of rats in all groups were compared,the control group＞ginsenoside Rg3 high dose group＞ginsenoside Rg3 low dose group＞model group,with statistical significance between any two groups(P＜0.05).The trabecular separation(TB.Sp)and ALP level of rats in all groups were compared,the control group＜ginsenoside Rg3 high dose group＜ginsenoside Rg3 low dose group＜model group,and the difference between any two groups was statistically significant(P＜0.05).Conclusion Ginsenoside Rg3 can improve bone metabolism,increase the levels of osteocalcin and oste-osin and improve osteoporosis in rats.The mechanism may be related to the regulation of RANKL/RANK/TRAF6 signaling pathway.
万方数据
维普
