Levels of NF1 and SKAP1 in endometrial carcinoma tissues and their relationship with clinicopathological features and survival
Objective To investigate the levels of neurofibromatosis gene(NF1)and kinase-associated phosphoprotein 1(SKAP1)in endometrial carcinoma tissues and their relationship with clinicopathological features and survival.Methods A total of 89 patients with endometrial cancer who underwent surgical treat-ment in the gynecology department of this hospital from January 2018 to December 2018 were selected as the study group,and 80 patients who underwent total hysterectomy due to uterine fibroids or uterine prolapse in this hospital during the same period were selected as the control group.The levels of NF1 and SKAP1 messen-ger RNA(mRNA)and the positive expression of NF1 and SKAP1 proteins in endometrial carcinoma tissues and normal tissues were detected.Pearson correlation was used to analyze the correlation between NF1 mRNA and SKAP1 mRNA levels in endometrial carcinoma tissues.Kaplan-Meier method was used to analyze the re-lationship between NF1 and SKAP1 levels and survival.Results The level of NF1 mRNA in the study group was lower than that in the control group,and the level of SKAP1 mRNA was higher than that in the control group,with statistical significance(P＜0.001).NF1 and SKAP1 proteins were mainly located in cytoplasm and cell membrane.The positive expression rate of NF1 protein in endometrial carcinoma tissues was lower than that in normal tissues,and the positive expression rate of SKAP1 protein was higher than that in normal tissues,with statistical significance(P＜0.001).Pearson correlation analysis showed that NF1 mRNA levels were negatively correlated with SKAP1 mRNA levels in endometrial carcinoma tissues(r=-0.253,P=0.017).There were statistically significant differences in the positive expression of NF1 protein and SKAP1 protein in endometrial carcinoma tissues of patients with different muscle infiltration depth,differentiation de-gree,international federation of gynecology and obstetrics(FIGO)stage,lymph node metastasis,and vascular cancer thromboma(P＜0.05).Kaplan-Meier analysis showed that the cumulative survival rate of patients with pos-itive expression of NF1 protein was higher than that of patients with negative expression of NF1 protein,and the cu-mulative survival rate of patients with positive expression of SKAP1 protein was lower than that of patients with nega-tive expression of SKAP1 protein,with statistical significance(P＜0.05).Conclusion NF1 expression is down-regulated and SKAP1 expression is up-regulated in endometrial carcinoma tissues,and the two expressions are negatively correlated with the depth of muscular invasion,degree of differentiation,FIGO stage,lymph node metastasis,and vascular cancer embolus,which may be used as prognostic markers for endometrial carcinoma patients.
endometrial carcinomaneurofibroma genekinase-associated phosphoprotein 1survival ratedepth of infiltration
万方数据
维普
