Objective To investigate whether small extracellular vesicles(sEVs)secreted by sensory neurons in the dorsal root ganglion(DRG)can affect ossification of posterior longitudinal ligament(OPLL)cells in vitro.Methods Ligament cells were extracted and identified from ligament tissues obtained from surgical specimens of patients with OPLL.The sEVs secreted by sensory neurons in the DRG were extracted,purified and identified,and then co-cultured with ligament cells as a stimulus.The ligament cell proliferation was detected by CCK8,the effect of sEVs on the vitality of the ligament cells determined.The sEVs were added to the ligament cells during the osteogenic differentiation process,to observe their effect on the osteogenic differentiation of ligament cells,and the expression of the osteogenic markers of runt-related transcription factor 2(RUNX2)and osteocalcin(OCN)were detected.Results In DRG,sensory neurons could secrete sEVs.After stimulation with sEVs,the proliferation ability of posterior longitudinal ligament cells significantly increased.During the process of osteogenic differentiation,sEVs could promote the osteogenic differentiation of posterior longitudinal ligament cells and promote the expression of osteogenic markers RUNX2 and OCN.Conclusion The sEVs secreted by sensory neurons in DRG can promote the ossification of posterior longitudinal ligament cells.
维普
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