Study on Lactobacillus casei ATCC334 Inhibiting Inflammatory Response in Collagen-induced Arthritis Rats
Objective To explore whether Lactobacillus casei ATCC334 can inhibit the inflammatory response of collagen-in-duced arthritis(CIA)rats and whether the NF-κB signaling pathway is involved in this process.Methods 21 Wistar rats were ran-domly divided into three groups:A was the normal control group,B was the CIA group,and C was the Lactobacillus casei ATCC334 group.Treatment factors in group A were as follows:(1)In control group,normal saline was given intragastric administration,500 μL daily,for 28 consecutive days without interruption;(2)in CIA group,at the same time of modeling,normal saline was adminis-tered by gavage,500 μL daily,for 28 consecutive days;(3)in ATCC334 group,at the same time of modeling,500 μL of Lacto-bacillus casei ATCC334 solution was injected into the stomach at a concentration of 2×108 CFU/mL every day for 28 consecutive days.The growth volume of the hind foot of the rats was measured using paw and toe volume instrument,and the rats were killed on day 29.Serum levels of TNF-α and IL-6 were detected by ELISA,and CD4+T cells were isolated by single-cell suspension from popliteal and inguinal lymph nodes.The levels of TNF-α,IL-6 and NF-κB proteins in CD4+T cells were detected by Western Blot.Results Lactobacillus casei ATCC334 reduced the arthritis index and hind paw growth volume of CIA rats,reduced the serum levels of-α and IL-6 in CIA rats,and reduced the expression of NF-κB p65 protein in CD4+T cells of CIA rats.Conclusion Lactobacillus casei ATCC334 has the effect of anti-arthritis and inhibits inflammatory factors,and NF-κB may be involved in this process.
rheumatoid arthritisLactobacillus casei ATCC334NF-κBTNF-αIL-6
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