Treatment for Gouty Arthritis by Regulating Macrophage Polarization with Gancao Fuzi Decoction
Objective To investigate the effect of Gancao Fuzi Decoction on gouty arthritis induced by sodium urate crystals in rats,and to further investigate the potential mechanism of Gancao Fuzi Decoction by regulating the polarization pathway of macropha-ges.Methods A total of 40 SD rats were included in this experiment and randomly assigned into control group,model group,colchi-cine group and Gancao Fuzi Decoction group.MSU was injected into the ankle cavity of rats to construct an animal model of gouty ar-thritis.The severity of symptoms was assessed by foot swelling and gait changes in rats;HE staining technique was used to score the joint tissue samples;plasma IL-1β and IL-10 were determined by enzyme-linked immunosorbent assay(ELISA);the expression of iNOS and Arg-1 in the joints of rats was determined by Real-Time PCR;the expression of TNF-α in synovial tissue was determined by immunofluorescence.Results Gancao Fuzi Decoction can significantly reduce the swelling of foot and plantar of rats with gouty ar-thritis,and improve gait and histological score(P<0.05).After treatment with Gancao Fuzi Decoction,serum IL-1β expression lev-el was significantly decreased(P<0.05),the expression level of IL-10,however,was significantly increased(P<0.05);at the same time,Real-Time PCR results showed that the expression of iNOS was significantly decreased(P<0.01)and the expression of Arg-1 was increased(P<0.01);meanwhile,immunofluorescence detection showed that Gancao Decoction inhibited the expression of TNF-α.Conclusion Gancao Fuzi Decoction demonstrates a pronounced suppressive impact on gouty arthritis.This effect is likely associated with its role in facilitating the transition of macrophages from the M1 to the M2 phenotype.
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