爱必妥对前列腺癌PC-3细胞功能学的影响
Study on the Functional Effects of Cetuximab on Prostate Cancer PC-3 Cells
于卓玄 1姜树旭 1李昶毅 1何东岳 1赫振 1傅德望1
作者信息
- 1. 锦州医科大学附属第一医院泌尿外科,辽宁 锦州 121000
- 折叠
摘要
目的 观察不同浓度爱必妥对前列腺癌PC-3 细胞的增殖、侵袭及凋亡的作用,并初步探索其可能的作用机制.方法 使用不同浓度的(10、20、40、80 μg/mL)爱必妥作用于PC-3 细胞24h后,CCK-8 检测细胞增殖活力变化;流式检测细胞凋亡变化;transwell检测细胞侵袭能力变化;RT-PCR检测EGFR mRNA表达变化;Western Blot法检测Bax和Bcl-2 蛋白表达变化.结果 CCK-8 结果显示,不同浓度的爱必妥作用24h后均可以抑制PC-3 的细胞增殖活力(P<0.001),且药物抑制作用与浓度呈剂量依赖关系;凋亡检测结果显示,不同浓度的爱必妥均抑制 PC-3 细胞的侵袭能力(P<0.001),且药物抑制作用与浓度呈剂量依赖关系;凋亡检测结果显示,不同浓度的爱必妥均导致PC-3 细胞的总凋亡比例增加(P<0.01),且这种促凋亡能力与与浓度呈剂量依赖关系;Western Blot结果显示,不同浓度的爱必妥均可以抑制Bc1-2 蛋白表达(P<0.05),同时促进Bax蛋白表达(P<0.05),且抑制和促进作用与药物浓度呈剂量依赖关系;RT-PCR结果显示,不同浓度的爱必妥均可以抑制EGFR mRNA表达(P<0.05),且抑制作用与药物浓度同样呈剂量依赖关系.结论 爱必妥可以抑制前列腺癌细胞PC-3 的增殖和侵袭,促进细胞凋亡,且这种作用可能是通过抑制EGFR信号通路导致细胞凋亡而实现.
Abstract
Objective To observe the effects of different concentrations of cetuximab on the proliferation,invasion and apoptosis of prostate cancer PC-3 cells,and to explore its possible mechanism.Methods PC-3 cells were treated with different concentrations of cetuximab(10,20,40,and 80 μg/mL)for 24 h,and CCK-8 was used to detect changes in cell proliferation activity;flow cy-tometry was used to detect changes in cell apoptosis;transwell assay was used to detect the changes in cell invasion ability;RT-PCR was used to detect changes in EGFR mRNA expression;Western Blot was used to detect changes in Bax and Bcl-2 protein expression.Results The CCK-8 results showed that different concentrations of cetuximab could inhibit the cell proliferation activity of PC-3 after 24 hours of action(P<0.001),and the drug's inhibitory effect was dose-dependent on the concentration;the results of apoptosis de-tection showed that different concentrations of cetuximab inhibited the invasive ability of PC-3 cells(P<0.001),and the drug's in-hibitory effect was dose-dependent on the concentration;the apoptosis detection results showed that different concentrations of cetux-imab resulted in an increase in the total apoptosis proportion of PC-3 cells(P<0.01),and this proapoptotic ability was dose-de-pendent on the concentration;Western Blot results showed that different concentrations of cetuximab could inhibit Bc1-2 protein ex-pression(P<0.05)and promote Bax protein expression(P<0.05),and the inhibitory and promoting effects were dose-dependent on drug concentration;the RT-PCR results showed that different concentrations of cetuximab could inhibit EGFR mRNA expression(P<0.05),and the inhibitory effect was also dose-dependent on the drug concentration.Conclusion Cetuximab can inhibit the pro-liferation and invasion of PC-3 in prostate cancer cells and promote cell apoptosis,and this effect may be achieved by inhibiting EGFR signaling pathway leading to cell apoptosis.
关键词
前列腺癌/PC-3/爱必妥/EGFR/凋亡Key words
prostatic cancer/PC-3/cetuximab/EGFR/apoptosis引用本文复制引用
基金项目
锦州医科大学大学生创新创业训练计划(x202210160076)
出版年
2024
NETL
NSTL
万方数据