Construction of Tissue Engineering Urethra Modified with Vascular Endothelial Factor for Repairing Urethral Defects in Rats
Objective To investigate the effect of vascular endothelial factor modified tissue engineering urethra on repairing ure-thral defects in rats.Methods A total of 20 SD rat urethra segments,each 1.5 cm long,were taken.The cellular components of the rat urethra were treated with chemical acellular method,while the extracellular matrix was preserved to prepare tissue engineered ure-thra.Ten Wistar rats were used to create a 1.5 cm complete segment defect model of the urethra,and SD rats were repaired with tissue engineering urethra modified with vascular endothelial factor(treatment group).Ten Wistar rats were used to create a 1.5 cm com-plete segment defect model of the urethra,and SD rat acellular allogeneic urethral transplantation was used to repair the entire segment defect of the Wistar rat urethra(control group).Results Compared with the control group,the VG staining results showed that the broken end of the urethral anastomosis in the treatment group was dyed red,and the muscle fibers were arranged neatly and evenly,with a large amount of regenerated muscle fibers growing into the distal urethra.The morphology of the anastomotic muscle fibers in the control group is uniform,and the muscle fibers are arranged neatly and evenly distributed.A small amount of muscle fibers can be seen growing into the distal urethra.The HE staining results showed that blood vessels of varying sizes grew into the acellular urethra in the treatment group,with some tending to form blood sinuses;however,the control group did not show any absorption of the urethra or tendency towards angiogenesis.The electrophysiological test results showed that the electrode stimulated the S1 nerve.Treatment group:Latency(1.62±0.47)ms,amplitude(1.11±0.67)mv.Control group:Latency(2.31±0.17)ms,amplitude(2.51±0.23)mv.The tissue engineering urethra modified with vascular endothelial factor has the advantages of weak immunogenicity,com-plete removal of cell membrane fragments,and intact preservation of the urethral basement membrane.Conclusion Using the acellu-lar urethra of mice as a matrix,constructing a tissue engineering urethra can repair a 1.5 cm long complete urethral defect in mice,reduce the incidence of complications,and obtain good mucosal coverage after surgery,forming a tissue structure similar to that of a normal urethra.
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