Protective Effect and Mechanism of Catalpol on Rats with Diabetic Cardiomyopathy Through NLRP3/Caspase-1 Pathway
Objective To investigate the protective effect of catalpol on the heart of diabetic cardiomyopathy rats through NL-RP3/Caspase-1 pathway and its mechanism.Methods 70 heacthy SD rats randomly selected from 80 healthy SD rats were fed high-fat and high-sugar diet,and were injected peritoneally with streptozotocin to establish diabetic rat model,and the remaining 10 were fed normal diet as control group.A total of 60 successful rats were randomly divided into model group,catalpol low-dose group,catalpol medium-dose group and catalpol high-dose group(15 rats in each group).The low,medium and high dose groups of catal-pol were given 100 mg/kg,300 mg/kg and 900 mg/kg intragastatically once a day for 8 weeks,during which high fat and high sugar feeding was maintained;the control group and model group were given normal diet and normal saline.After the last gavage,the cardi-ac function of rats was detected by ultrasound,the myocardial morphological changes were observed by HE staining,and the protein expressions of NLRP3 and Caspase-1 in myocardial tissue were detected by Western Blot.The contents of interleukin-1 β(IL-1 β)and interleukin-18(IL-18)in serum of rats were detected by enzyme-linked immunosorbent assay(ELISA).Results The left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(FS)in the model group exhibited a significant de-crease compared to the control group(P＜0.05).HE staining showed swelling,disordered arrangement of myocardial fibers and wide-ning of fiber bundle interval.In myocardial tissue,the expression of Caspase-1 and NLRP3 proteins were significantly upregulated,accompanied by a marked increase in the concentrations of IL-1β and IL-18 in serum(P＜0.05).Compared with the model group,LVEF and FS in low,medium and high dose groups of catalpa were significantly improved(P＜0.05),the pathological injury of myo-cardium was significantly improved,and the protein expressions of NLRP3 and Caspase-1 in myocardium and the contents of IL-1 βand IL-18 in serum were significantly decreased(P＜0.05).In high dose group of catalpol,the improvement was more significant.Conclusion Catalpol protects myocardium in DCM rats by regulating NLRP3/Caspase-1 signaling pathway and inhibiting scorch death of myocardium in DCM rats.
万方数据
维普
