Berberine Promotes Neural Function Recovery in Cerebral Ischemia-reperfusion Rats by Regulating the TLR4/p38 MAPK Pathway
Objective To observe the neuroprotective effect of berberine on rats with cerebral ischemia-reperfusion injury and explore its mechanism of action.Methods 45 SD rats were randomly divided into three groups:sham surgery group(sham),model group(MCAO/R),and berberine treatment group(Ber).A cerebral ischemia-reperfusion model was established using the suture method.The dosage of Ber group was 140mg/kg.After 28 days of administration,Zea-longa score and rotating rod experiment were used to evaluate the neural function of rats.After 24 hours of administration,the infarct area was observed and calculated through TTC staining.After 24 hours of administration,brain tissue samples were taken from rats.Immunofluorescence staining was used to ob-serve the expression of interleukin 1β(IL-1 β),enzyme linked immunosorbent assay(ELISA)was used to detect the content of IL-1β,tumor necrosis factor-α(TNF-α),interleukin 6(IL-6)and interleukin-10(IL-10),and Western Blot was used to detect the protein contents of TLR4,P-p38MAPK and p38MAPK in brain tissues of each group.Results The results of the Zea-longa score and the rotating rod experiment showed that berberine significantly improved the motor ability of rats with cerebral ischemia-reperfusion injury.The TTC staining results showed that berberine reduced the infarct area.The results of immunofluorescence staining and ELISA experiments confirm that berberine can reduce the pro-inflammatory factor IL-1 β、TNF-α、IL-6,and increases the con-tent of anti-inflammatory factor IL-10.The Western Blot results suggest that berberine affects the expression of TLR4/p38MAPK path-way proteins.Conclusion Berberine inhibits the secretion of inflammatory factors in the brain tissue of MCAO/R rats through the TLR4/p38MAPK signaling pathway,promoting the recovery of neurological function in ischemia-reperfusion rats.
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