Objective To observe the effect of curcumin on mitochondrial damage in renal tubular epithelial cells under high glu-cose,and to explore the action mechanism.Methods Human proximal renal tubular epithelial cells were divided into control group,high-glucose model group,and high-glucose+curcumin-treated group.The expression of PINK 1,Parkin,LC3Ⅱ,and Caspase-3 was determined by Western Blot,and mtROS expression was measured by flow cytometry.Results The expressions of PINK1,Par-kin and LC3Ⅱ were decreased in HK-2 cells treated with high glucose,while the expressions of mtROS and apoptosis-related protein Caspase-3 were increased.Compared with the high glucose group,the expressions of PINK1,Parkin and LC3Ⅱ were increased after curcumin treatment.The expression of mtROS and Caspase-3 was decreased.Conclusion Curcumin may play a role in treating dia-betic kidney disease by regulating pinkl/parkin-mediated mitophagy.
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