Objective Bioinformatics analysis was used to screen and verify B cell-related genes(BRGs)in septic cardiomyop-athy(SCM),and construct their ceRNA network to explore the mechanism of action of BRGs in SCM.The aim is to find new targets for early diagnosis and treatment of SCM.Methods The data sets GSE79962 and GSE63920 in GEO database were used to screen common differential genes(DEGs),then their biological functions were analyzed by GO and KEGG,and the interaction between pro-teins was analyzed by STRING online tool.Core Differential Genes(Hub DEGs)were obstained via CytoScape's CytoHubba plugin a-nalysis.On the other hand,B-cell-related gene modules were obtained by immune cell infiltration analysis and WGCNA analysis,and B-cell-related hub genes(BRHGs)were obtained by intersection with Hub DEGs,and their expression was verified in related data sets.Finally,we constructed and validated B-cell-related ceRNA networks using online tools and public datasets.Results GSE79962 and GSE63920 selected 29 common DEGs;GO and KEGG enrichment analysis suggested that it plays a regulatory role mainly through cell migration,cell transport and negative regulation of cell component movement,and it is mainly involved in JAK-STAT signaling pathway;the results of immune cell infiltration showed that the content of B cells in SCM group was significantly lower than that in control group in GSE79962 dataset(P＜0.05).A total of 1,781 BRGs were selected by WGCNA and intersected with 10 Hub DEGs in CytoScape to obtain BRHG(TIMP1).The miRNA and lncRNA predicted by the online tool were let-7b-5p and KC-NQ1OT1,respectively,and the verification results of corresponding public data sets were statistically significant.B-cell-associated ceRNA network(KCNQ1OT1/let-7b-5p/TIMP1)was successfully constructed.Conclusion This study identified and validated BRHG(TIMP1)involved in the pathogenesis of SCM,further explored the possible molecular mechanism of B cell-mediated immune response in SCM,and provided a new molecular target for the early diagnosis and treatment of SCM.
septic cardiomyopathyB cell related genesceRNA network modelbioinformatics analysis
维普
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